Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides

T. N. Seyfried; E. J. Weber; R. K. Yu

doi:10.1007/BF02533321

Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides

T. N. Seyfried, E. J. Weber, R. K. Yu

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9 Scopus citations

Abstract

Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.

Original language	English (US)
Pages (from-to)	979-980
Number of pages	2
Journal	Lipids
Volume	12
Issue number	11
DOIs	https://doi.org/10.1007/BF02533321
State	Published - Nov 1 1977
Externally published	Yes

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ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Cell Biology

Cite this

Seyfried, T. N., Weber, E. J., & Yu, R. K. (1977). Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides. Lipids, 12(11), 979-980. https://doi.org/10.1007/BF02533321

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AB - Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.

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10.1007/BF02533321