Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides

T. N. Seyfried, E. J. Weber, Robert K Yu

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.

Original languageEnglish (US)
Pages (from-to)979-980
Number of pages2
JournalLipids
Volume12
Issue number11
DOIs
StatePublished - Nov 1 1977
Externally publishedYes

Fingerprint

Phosphotungstic Acid
phosphotungstic acid
gangliosides
Gangliosides
Trichloroacetic Acid
trichloroacetic acid
ethylenediamine
Acids
Dialysis
acids
dialysis
acid treatment
acid deposition
EDTA (chelating agent)
Artifacts
Hydrolysis
Salts
hydrolysis
salts

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

Cite this

Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides. / Seyfried, T. N.; Weber, E. J.; Yu, Robert K.

In: Lipids, Vol. 12, No. 11, 01.11.1977, p. 979-980.

Research output: Contribution to journalArticle

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