Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides

T. N. Seyfried, E. J. Weber, R. K. Yu

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.

Original languageEnglish (US)
Pages (from-to)979-980
Number of pages2
JournalLipids
Volume12
Issue number11
DOIs
StatePublished - Nov 1 1977
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology

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