Abstract
Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 979-980 |
| Number of pages | 2 |
| Journal | Lipids |
| Volume | 12 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1 1977 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry
- Cell Biology
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Cite this
Seyfried, T. N., Weber, E. J., & Yu, R. K. (1977). Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides. Lipids, 12(11), 979-980. https://doi.org/10.1007/BF02533321