Abstract
Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 979-980 |
| Number of pages | 2 |
| Journal | Lipids |
| Volume | 12 |
| Issue number | 11 |
| DOIs | |
| State | Published - Nov 1 1977 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry
- Cell Biology
Cite this
Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides. / Seyfried, T. N.; Weber, E. J.; Yu, Robert K.
In: Lipids, Vol. 12, No. 11, 01.11.1977, p. 979-980.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of trichloracetic acid-phosphotungstic acid on the thin layer chromatographic mobility of gangliosides
AU - Seyfried, T. N.
AU - Weber, E. J.
AU - Yu, Robert K
PY - 1977/11/1
Y1 - 1977/11/1
N2 - Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.
AB - Trichloroacetic acid (TCA)-phosphotungstic acid (PTA) precipitation has been used as a faster procedure than dialysis for the isolation of gangliosides, but the TCA-PTA treatment causes striking abnormalities in the thin layer chromatographic mobilities of the gangliosides. However, a normal chromatographic pattern can be restored by treating the precipitated gangliosides with the tetrasodium salt of ethylenediamine tetraacetic acid followed by dialysis. Hence, TCA-PTA treatment does not appear to cause artifacts or hydrolysis of the gangliosides.
UR - http://www.scopus.com/inward/record.url?scp=0017669179&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017669179&partnerID=8YFLogxK
U2 - 10.1007/BF02533321
DO - 10.1007/BF02533321
M3 - Article
C2 - 927050
AN - SCOPUS:0017669179
VL - 12
SP - 979
EP - 980
JO - Lipids
JF - Lipids
SN - 0024-4201
IS - 11
ER -