Infrared fluorescent protein 1.4 genetic labeling tracks engrafted cardiac progenitor cells in mouse ischemic hearts

Lijuan Chen, M. Ian Phillips, Hui Lai Miao, Rong Zeng, Gangjian Qin, Il-man Kim, Neal Lee Weintraub, Yao Liang Tang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Stem cell therapy has a potential for regenerating damaged myocardium. However, a key obstacle to cell therapy's success is the loss of engrafted cells due to apoptosis or necrosis in the ischemic myocardium. While many strategies have been developed to improve engrafted cell survival, tools to evaluate cell efficacy within the body are limited. Traditional genetic labeling tools, such as GFP-like fluorescent proteins (eGFP, DsRed, mCherry), have limited penetration depths in vivo due to tissue scattering and absorption. To circumvent these limitations, a near-infrared fluorescent mutant of the DrBphP bacteriophytochrome from Deinococcus radiodurans, IFP1.4, was developed for in vivo imaging, but it has yet to be used for in vivo stem/progenitor cell tracking. In this study, we incorporated IFP1.4 into mouse cardiac progenitor cells (CPCs) by a lentiviral vector. Live IFP1.4-labeled CPCs were imaged by their near-infrared fluorescence (NIRF) using an Odyssey scanner following overnight incubation with biliverdin. A significant linear correlation was observed between the amount of cells and NIRF signal intensity in in vitro studies. Lentiviral mediated IFP1.4 gene labeling is stable, and does not impact the apoptosis and cardiac differentiation of CPC. To assess efficacy of our model for engrafted cells in vivo, IFP1.4-labeled CPCs were intramyocardially injected into infarcted hearts. NIRF signals were collected at 1-day, 7-days, and 14-days postinjection using the Kodak in vivo multispectral imaging system. Strong NIRF signals from engrafted cells were imaged 1 day after injection. At 1 week after injection, 70% of the NIRF signal was lost when compared to the intensity of the day 1 signal. The data collected 2 weeks following transplantation showed an 88% decrease when compared to day 1. Our studies have shown that IFP1.4 gene labeling can be used to track the viability of transplanted cells in vivo.

Original languageEnglish (US)
Article numbere107841
JournalPloS one
Volume9
Issue number10
DOIs
StatePublished - Oct 30 2014

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Labeling
stem cells
Stem Cells
heart
Infrared radiation
Fluorescence
mice
fluorescence
Proteins
Stem cells
cells
Cell- and Tissue-Based Therapy
myocardium
cell viability
Genes
Cells
Cell Survival
Myocardium
Biliverdine
Apoptosis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Infrared fluorescent protein 1.4 genetic labeling tracks engrafted cardiac progenitor cells in mouse ischemic hearts. / Chen, Lijuan; Phillips, M. Ian; Miao, Hui Lai; Zeng, Rong; Qin, Gangjian; Kim, Il-man; Weintraub, Neal Lee; Tang, Yao Liang.

In: PloS one, Vol. 9, No. 10, e107841, 30.10.2014.

Research output: Contribution to journalArticle

Chen, Lijuan ; Phillips, M. Ian ; Miao, Hui Lai ; Zeng, Rong ; Qin, Gangjian ; Kim, Il-man ; Weintraub, Neal Lee ; Tang, Yao Liang. / Infrared fluorescent protein 1.4 genetic labeling tracks engrafted cardiac progenitor cells in mouse ischemic hearts. In: PloS one. 2014 ; Vol. 9, No. 10.
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