Ingestion of bacterial lipopolysaccharide inhibits peripheral taste responses to sucrose in mice

X. Zhu, L. He, Lynnette McCluskey

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A fundamental role of the taste system is to discriminate between nutritive and toxic foods. However, it is unknown whether bacterial pathogens that might contaminate food and water modulate the transmission of taste input to the brain. We hypothesized that exogenous, bacterially-derived lipopolysaccharide (LPS), modulates neural responses to taste stimuli. Neurophysiological responses from the chorda tympani nerve, which innervates taste cells on the anterior tongue, were unchanged by acute exposure to LPS. Instead, neural responses to sucrose were selectively inhibited in mice that drank LPS during a single overnight period. Decreased sucrose sensitivity appeared 7. days after LPS ingestion, in parallel with decreased lingual expression of Tas1r2 and Tas1r3 transcripts, which are translated to T1R2. +. T1R3 subunits forming the sweet taste receptor. Tas1r2 and Tas1r3 mRNA expression levels and neural responses to sucrose were restored by 14. days after LPS consumption. Ingestion of LPS, rather than contact with taste receptor cells, appears to be necessary to suppress sucrose responses. Furthermore, mice lacking the Toll-like receptor (TLR) 4 for LPS were resistant to neurophysiological changes following LPS consumption. These findings demonstrate that ingestion of LPS during a single period specifically and transiently inhibits neural responses to sucrose. We suggest that LPS drinking initiates TLR4-dependent hormonal signals that downregulate sweet taste receptor genes in taste buds. Delayed inhibition of sweet taste signaling may influence food selection and the complex interplay between gastrointestinal bacteria and obesity.

Original languageEnglish (US)
Pages (from-to)47-61
Number of pages15
JournalNeuroscience
Volume258
DOIs
StatePublished - Jan 31 2014

Fingerprint

Lipopolysaccharides
Sucrose
Eating
Tongue
Chorda Tympani Nerve
Taste Buds
Food Preferences
Food
Poisons
Drinking
Down-Regulation
Obesity
Bacteria
Messenger RNA
Water
Brain
Genes

Keywords

  • Chorda tympani nerve
  • Neuro-immune interactions
  • Tas1r1
  • Tas1r2
  • Tas1r3
  • Taste receptor cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ingestion of bacterial lipopolysaccharide inhibits peripheral taste responses to sucrose in mice. / Zhu, X.; He, L.; McCluskey, Lynnette.

In: Neuroscience, Vol. 258, 31.01.2014, p. 47-61.

Research output: Contribution to journalArticle

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