Inherent physical characteristics and gene expression differences between alveolar and basal bones

Ibrahim Zakhary, Fawwaz Alotibi, Jill Lewis, Mohammed Elsayed Elsalanty, Karl Wenger, Mohamed M.H. Sharawy, Regina L W Messer

Research output: Contribution to journalArticle

Abstract

Objectives The objective of this study was to evaluate the baseline differences between alveolar and basal areas of the rat mandible. Study Design Rat mandibular alveolar and basal bones were evaluated using histology and micro-computed tomography to compare osteocyte number as well as bone density and architecture and polymerase chain reaction to measure gene expression levels. Results Micro-computed tomography data indicated that basal bone is denser and less porous than alveolar bone. Histologic analysis showed that alveolar bone has more osteocytes per unit area compared with basal bone. Real-time polymerase chain reaction results showed higher levels of expression of the following genes in basal bone than in alveolar bone: SOST, E-11, DMP-1, and MEPE. Conclusions Three of these gene products are associated with mature osteocytes, and this suggests that basal bone has more mature osteocyte phenotypes compared with alveolar bone. These findings are suggestive of fewer bone mineralization units and therefore a slower remodeling rate.

Original languageEnglish (US)
Pages (from-to)35-42
Number of pages8
JournalOral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Volume122
Issue number1
DOIs
StatePublished - Jul 1 2016

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Gene Expression
Bone and Bones
Osteocytes
Tomography
Physiologic Calcification
Mandible
Bone Density
Real-Time Polymerase Chain Reaction
Histology
Phenotype
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Surgery
  • Oral Surgery
  • Pathology and Forensic Medicine
  • Dentistry (miscellaneous)
  • Radiology Nuclear Medicine and imaging

Cite this

Inherent physical characteristics and gene expression differences between alveolar and basal bones. / Zakhary, Ibrahim; Alotibi, Fawwaz; Lewis, Jill; Elsalanty, Mohammed Elsayed; Wenger, Karl; Sharawy, Mohamed M.H.; Messer, Regina L W.

In: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology, Vol. 122, No. 1, 01.07.2016, p. 35-42.

Research output: Contribution to journalArticle

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AU - Sharawy, Mohamed M.H.

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