Inhibition by the JAK/STAT pathway of IFNγ- and LPS-stimulated nitric oxide synthase induction in vascular smooth muscle cells

Mario B. Marrero, Virginia J. Venema, Han He, Ruth B Caldwell, Richard C. Venema

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Inducible nitric oxide synthase (iNOS) is induced in many cell types by cytokines and lipopolysaccharide (LPS). Cytokine signal transduction is believed to be mediated primarily through the JAK/STAT pathway. We therefore examined the effects of a JAK2-specific inhibitor, an antisense oligonucleotide to JAK2, and electroporation of neutralizing anti-STAT1 and anti-STAT3 antibodies on IFNγ- and LPS-stimulated induction of iNOS in vascular smooth muscle cells. Unexpectedly, we found that the JAK/STAT pathway suppresses IFNγ- and LPS-stimulated iNOS induction in these cells. In contrast, the JAK/STAT pathway appears to have a positive role in iNOS induction in RAW 264.7 macrophages.

Original languageEnglish (US)
Pages (from-to)508-512
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume252
Issue number2
DOIs
StatePublished - Nov 18 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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