Inhibition of apoptosis by taurine in macrophages treated with sodium nitroprusside

So Young Kim, Taesun Park, Ha Won Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Nitric oxide (NO) induces apoptotic cell death in murine RAW264.7 macrophages. To elucidate the mechanism underlying the inhibitory effect of taurine on NO-induced apoptosis, a cell was exposed to sodium nitroprusside (SNP), an NO donor, in the absence and presence of taurine. Taurine treatment prevented SNP-mediated cellular apoptosis in a concentration dependent manner. The exposure of the cell to taurine prior to SNP treatment inhibited DNA fragmentation more than addition of taurine to the medium after SNP treatment. Agarose gel electrophoresis data revealed that taurine reduced the intensity of SNP-induced DNA laddering. The taurine-mediated reduction in the number of apoptotic cells was also observed using the Hoechst 33258 stain. These results support the idea that taurine has the potential to function as an inhibitory modulator of NO-mediated cell injury.

Original languageEnglish (US)
Title of host publicationTaurine 7
Pages481-489
Number of pages9
DOIs
Publication statusPublished - Apr 22 2009
Externally publishedYes

Publication series

NameAdvances in Experimental Medicine and Biology
Volume643
ISSN (Print)0065-2598

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kim, S. Y., Park, T., & Kim, H. W. (2009). Inhibition of apoptosis by taurine in macrophages treated with sodium nitroprusside. In Taurine 7 (pp. 481-489). (Advances in Experimental Medicine and Biology; Vol. 643). https://doi.org/10.1007/978-0-387-75681-3_50