Inhibition of endogenous human dentin MMPs by Gluma

Camila Sabatini, Débora L.S. Scheffel, Régis H. Scheffel, Kelli A. Agee, Katelyn Rouch, Masahiro Takahashi, Lorenzo Breschi, Annalisa Mazzoni, Leo Tjäderhane, Franklin R. Tay, David H. Pashley

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective The objective of this study was to determine if Gluma dentin desensitizer (5.0% glutaraldehyde and 35% HEMA in water) can inhibit the endogenous MMPs of dentin matrices in 60 s and to evaluate its effect on dentin matrix stiffness and dry mass weight. Methods Dentin beams of 2 mm × 1 mm × 6 mm were obtained from extracted human third molars coronal dentin. To measure the influence of Gluma treatment time on total MMP activity of dentin, beams were dipped in 37% phosphoric acid (PA) for 15 s and rinsed in water. The acid-etched beams were then dipped in Gluma for 5, 15, 30 or 60 s, rinsed in water and incubated into SensoLyte generic MMP substrate (AnaSpec, Inc.) for 60 min. Controls were dipped in water for 60 s. Additional beams of 1 mm × 1 mm × 6 mm were completely demineralized in 37% PA for 18 h, rinsed and used to evaluate changes on the dry weight and modulus of elasticity (E) after 60 s of Gluma treatment followed by incubation in simulated body fluid buffer for 0, 1 or 4 weeks. E was measured by 3-pt flexure. Results Gluma treatment inhibited total MMP activity of acid-etched dentin by 44, 50, 84, 86% after 5, 15, 30 or 60 s of exposure, respectively. All completely demineralized dentin beams lost stiffness after 1 and 4 weeks, with no significant differences between the control and Gluma-treated dentin. Gluma treatment for 60 s yielded significantly less dry mass loss than the control after 4 weeks. Significance The use of Gluma may contribute to the preservation of adhesive interfaces by its cross-linking and inhibitory properties of endogenous dentin MMPs.

Original languageEnglish (US)
Pages (from-to)752-758
Number of pages7
JournalDental Materials
Volume30
Issue number7
DOIs
StatePublished - Jul 2014

Fingerprint

Dentin
Matrix Metalloproteinases
Phosphoric acid
Water
Acids
Body fluids
Stiffness matrix
Adhesives
Elastic moduli
Stiffness
Glutaral
Gluma
Weights and Measures
Third Molar
Substrates
Elastic Modulus
Body Fluids
Buffers

Keywords

  • Degradation
  • Dentin collagen
  • Glutaraldehyde
  • Matrix metalloproteinase
  • Mechanical properties
  • Proteolytic activity

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Sabatini, C., Scheffel, D. L. S., Scheffel, R. H., Agee, K. A., Rouch, K., Takahashi, M., ... Pashley, D. H. (2014). Inhibition of endogenous human dentin MMPs by Gluma. Dental Materials, 30(7), 752-758. https://doi.org/10.1016/j.dental.2014.04.006

Inhibition of endogenous human dentin MMPs by Gluma. / Sabatini, Camila; Scheffel, Débora L.S.; Scheffel, Régis H.; Agee, Kelli A.; Rouch, Katelyn; Takahashi, Masahiro; Breschi, Lorenzo; Mazzoni, Annalisa; Tjäderhane, Leo; Tay, Franklin R.; Pashley, David H.

In: Dental Materials, Vol. 30, No. 7, 07.2014, p. 752-758.

Research output: Contribution to journalArticle

Sabatini, C, Scheffel, DLS, Scheffel, RH, Agee, KA, Rouch, K, Takahashi, M, Breschi, L, Mazzoni, A, Tjäderhane, L, Tay, FR & Pashley, DH 2014, 'Inhibition of endogenous human dentin MMPs by Gluma', Dental Materials, vol. 30, no. 7, pp. 752-758. https://doi.org/10.1016/j.dental.2014.04.006
Sabatini C, Scheffel DLS, Scheffel RH, Agee KA, Rouch K, Takahashi M et al. Inhibition of endogenous human dentin MMPs by Gluma. Dental Materials. 2014 Jul;30(7):752-758. https://doi.org/10.1016/j.dental.2014.04.006
Sabatini, Camila ; Scheffel, Débora L.S. ; Scheffel, Régis H. ; Agee, Kelli A. ; Rouch, Katelyn ; Takahashi, Masahiro ; Breschi, Lorenzo ; Mazzoni, Annalisa ; Tjäderhane, Leo ; Tay, Franklin R. ; Pashley, David H. / Inhibition of endogenous human dentin MMPs by Gluma. In: Dental Materials. 2014 ; Vol. 30, No. 7. pp. 752-758.
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abstract = "Objective The objective of this study was to determine if Gluma dentin desensitizer (5.0{\%} glutaraldehyde and 35{\%} HEMA in water) can inhibit the endogenous MMPs of dentin matrices in 60 s and to evaluate its effect on dentin matrix stiffness and dry mass weight. Methods Dentin beams of 2 mm × 1 mm × 6 mm were obtained from extracted human third molars coronal dentin. To measure the influence of Gluma treatment time on total MMP activity of dentin, beams were dipped in 37{\%} phosphoric acid (PA) for 15 s and rinsed in water. The acid-etched beams were then dipped in Gluma for 5, 15, 30 or 60 s, rinsed in water and incubated into SensoLyte generic MMP substrate (AnaSpec, Inc.) for 60 min. Controls were dipped in water for 60 s. Additional beams of 1 mm × 1 mm × 6 mm were completely demineralized in 37{\%} PA for 18 h, rinsed and used to evaluate changes on the dry weight and modulus of elasticity (E) after 60 s of Gluma treatment followed by incubation in simulated body fluid buffer for 0, 1 or 4 weeks. E was measured by 3-pt flexure. Results Gluma treatment inhibited total MMP activity of acid-etched dentin by 44, 50, 84, 86{\%} after 5, 15, 30 or 60 s of exposure, respectively. All completely demineralized dentin beams lost stiffness after 1 and 4 weeks, with no significant differences between the control and Gluma-treated dentin. Gluma treatment for 60 s yielded significantly less dry mass loss than the control after 4 weeks. Significance The use of Gluma may contribute to the preservation of adhesive interfaces by its cross-linking and inhibitory properties of endogenous dentin MMPs.",
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AU - Sabatini, Camila

AU - Scheffel, Débora L.S.

AU - Scheffel, Régis H.

AU - Agee, Kelli A.

AU - Rouch, Katelyn

AU - Takahashi, Masahiro

AU - Breschi, Lorenzo

AU - Mazzoni, Annalisa

AU - Tjäderhane, Leo

AU - Tay, Franklin R.

AU - Pashley, David H.

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N2 - Objective The objective of this study was to determine if Gluma dentin desensitizer (5.0% glutaraldehyde and 35% HEMA in water) can inhibit the endogenous MMPs of dentin matrices in 60 s and to evaluate its effect on dentin matrix stiffness and dry mass weight. Methods Dentin beams of 2 mm × 1 mm × 6 mm were obtained from extracted human third molars coronal dentin. To measure the influence of Gluma treatment time on total MMP activity of dentin, beams were dipped in 37% phosphoric acid (PA) for 15 s and rinsed in water. The acid-etched beams were then dipped in Gluma for 5, 15, 30 or 60 s, rinsed in water and incubated into SensoLyte generic MMP substrate (AnaSpec, Inc.) for 60 min. Controls were dipped in water for 60 s. Additional beams of 1 mm × 1 mm × 6 mm were completely demineralized in 37% PA for 18 h, rinsed and used to evaluate changes on the dry weight and modulus of elasticity (E) after 60 s of Gluma treatment followed by incubation in simulated body fluid buffer for 0, 1 or 4 weeks. E was measured by 3-pt flexure. Results Gluma treatment inhibited total MMP activity of acid-etched dentin by 44, 50, 84, 86% after 5, 15, 30 or 60 s of exposure, respectively. All completely demineralized dentin beams lost stiffness after 1 and 4 weeks, with no significant differences between the control and Gluma-treated dentin. Gluma treatment for 60 s yielded significantly less dry mass loss than the control after 4 weeks. Significance The use of Gluma may contribute to the preservation of adhesive interfaces by its cross-linking and inhibitory properties of endogenous dentin MMPs.

AB - Objective The objective of this study was to determine if Gluma dentin desensitizer (5.0% glutaraldehyde and 35% HEMA in water) can inhibit the endogenous MMPs of dentin matrices in 60 s and to evaluate its effect on dentin matrix stiffness and dry mass weight. Methods Dentin beams of 2 mm × 1 mm × 6 mm were obtained from extracted human third molars coronal dentin. To measure the influence of Gluma treatment time on total MMP activity of dentin, beams were dipped in 37% phosphoric acid (PA) for 15 s and rinsed in water. The acid-etched beams were then dipped in Gluma for 5, 15, 30 or 60 s, rinsed in water and incubated into SensoLyte generic MMP substrate (AnaSpec, Inc.) for 60 min. Controls were dipped in water for 60 s. Additional beams of 1 mm × 1 mm × 6 mm were completely demineralized in 37% PA for 18 h, rinsed and used to evaluate changes on the dry weight and modulus of elasticity (E) after 60 s of Gluma treatment followed by incubation in simulated body fluid buffer for 0, 1 or 4 weeks. E was measured by 3-pt flexure. Results Gluma treatment inhibited total MMP activity of acid-etched dentin by 44, 50, 84, 86% after 5, 15, 30 or 60 s of exposure, respectively. All completely demineralized dentin beams lost stiffness after 1 and 4 weeks, with no significant differences between the control and Gluma-treated dentin. Gluma treatment for 60 s yielded significantly less dry mass loss than the control after 4 weeks. Significance The use of Gluma may contribute to the preservation of adhesive interfaces by its cross-linking and inhibitory properties of endogenous dentin MMPs.

KW - Degradation

KW - Dentin collagen

KW - Glutaraldehyde

KW - Matrix metalloproteinase

KW - Mechanical properties

KW - Proteolytic activity

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