Inhibition of growth of cervical cancer cells using a dominant negative estrogen receptor gene

William W. Au, Salama Abdou-Salama, Ayman Al-Hendy

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Objective.: Estrogen stimulates human papilloma virus oncogene expression, promotes cervical cancer (CC) cell proliferation and prevents apoptosis. Therefore, blockage of estrogen function may have therapeutic application to CC. Methods.: CasKi CC cells were transfected with an adenovirus expressing a dominant negative estrogen receptor gene (Ad-ER-DN) and their responses were investigated by RT-PCR, Flow Cytometry and Western blot assays. Result.: Transfected cells showed disturbance of cell colony morphology, reduced HPV E6 and E7 mRNA, interruption of cell proliferation, reduced cyclin D1 protein and expression of apoptosis. Conclusion.: We report, for the first time, the use of Ad-ER-DN to block estrogen receptors which led to dramatic changes in CC cells that are consistent with the possible reactivation of cellular p53 and Rb function. Their reactivation most likely allowed the recognition of existing chromosome abnormalities as a serious stress signal and the initiation of a cascade of cellular events in response to the stress, including the activation of the core apoptotic machinery which led to self-destruction of the CC cells.

Original languageEnglish (US)
Pages (from-to)276-280
Number of pages5
JournalGynecologic Oncology
Volume104
Issue number2
DOIs
StatePublished - Feb 2007

Keywords

  • Cervical cancer
  • Cyclin D1
  • Dominant negative estrogen receptor gene
  • Estrogen
  • Estrogen receptor
  • Gene therapy
  • HPV
  • Molecular intervention

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynecology

Fingerprint Dive into the research topics of 'Inhibition of growth of cervical cancer cells using a dominant negative estrogen receptor gene'. Together they form a unique fingerprint.

  • Cite this