Inhibition of growth of cervical cancer cells using a dominant negative estrogen receptor gene

William W. Au, Salama Abdou-Salama, Ayman Al-Hendy

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective.: Estrogen stimulates human papilloma virus oncogene expression, promotes cervical cancer (CC) cell proliferation and prevents apoptosis. Therefore, blockage of estrogen function may have therapeutic application to CC. Methods.: CasKi CC cells were transfected with an adenovirus expressing a dominant negative estrogen receptor gene (Ad-ER-DN) and their responses were investigated by RT-PCR, Flow Cytometry and Western blot assays. Result.: Transfected cells showed disturbance of cell colony morphology, reduced HPV E6 and E7 mRNA, interruption of cell proliferation, reduced cyclin D1 protein and expression of apoptosis. Conclusion.: We report, for the first time, the use of Ad-ER-DN to block estrogen receptors which led to dramatic changes in CC cells that are consistent with the possible reactivation of cellular p53 and Rb function. Their reactivation most likely allowed the recognition of existing chromosome abnormalities as a serious stress signal and the initiation of a cascade of cellular events in response to the stress, including the activation of the core apoptotic machinery which led to self-destruction of the CC cells.

Original languageEnglish (US)
Pages (from-to)276-280
Number of pages5
JournalGynecologic Oncology
Volume104
Issue number2
DOIs
StatePublished - Feb 1 2007

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Uterine Cervical Neoplasms
Estrogen Receptors
Growth
Genes
Papillomaviridae
Estrogens
Cell Proliferation
Apoptosis
Cyclin D1
Oncogenes
Adenoviridae
Chromosome Aberrations
Flow Cytometry
Western Blotting
Polymerase Chain Reaction
Messenger RNA
Proteins
Therapeutics

Keywords

  • Cervical cancer
  • Cyclin D1
  • Dominant negative estrogen receptor gene
  • Estrogen
  • Estrogen receptor
  • Gene therapy
  • HPV
  • Molecular intervention

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Oncology

Cite this

Inhibition of growth of cervical cancer cells using a dominant negative estrogen receptor gene. / Au, William W.; Abdou-Salama, Salama; Al-Hendy, Ayman.

In: Gynecologic Oncology, Vol. 104, No. 2, 01.02.2007, p. 276-280.

Research output: Contribution to journalArticle

Au, William W. ; Abdou-Salama, Salama ; Al-Hendy, Ayman. / Inhibition of growth of cervical cancer cells using a dominant negative estrogen receptor gene. In: Gynecologic Oncology. 2007 ; Vol. 104, No. 2. pp. 276-280.
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