Abstract
Internalization of herpes simplex virus type 1 (HSV) KOS strain by HEp-2 cells was reversibly inhibited by pretreatment of cells with cytochalasins B and D. Internalization of virus following preincubation at 4° C and temperature shift to 37°C was normally preceded by a 5 to 8 min lag period and was complete within 20 to 30 min. A similar lag period followed HSV addition at 37°C. Cytochalasin D was fivefold more active on HSV entry than cytochalasin B, with 50% inhibition at 2 μM and 10 μM respectively. Inhibition was completely reversible, such that all cell-bound infectious virus was recovered upon removal of cytochalasin. In conjunction with previous reports, the activity of cytochalasin on HSV entry suggests that a change in cytoskeletal structure following virus attachment triggers a microfilament activity important for internalization of HSV by HEp-2 cells.
Original language | English (US) |
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Pages (from-to) | 1601-1605 |
Number of pages | 5 |
Journal | Journal of General Virology |
Volume | 66 |
Issue number | 7 |
DOIs | |
State | Published - 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Virology