Inhibition of herpes simplex virus type 1 penetration by cytochalasins B and D

K. S. Rosenthal, R. Perez, C. Hodnichak

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Internalization of herpes simplex virus type 1 (HSV) KOS strain by HEp-2 cells was reversibly inhibited by pretreatment of cells with cytochalasins B and D. Internalization of virus following preincubation at 4° C and temperature shift to 37°C was normally preceded by a 5 to 8 min lag period and was complete within 20 to 30 min. A similar lag period followed HSV addition at 37°C. Cytochalasin D was fivefold more active on HSV entry than cytochalasin B, with 50% inhibition at 2 μM and 10 μM respectively. Inhibition was completely reversible, such that all cell-bound infectious virus was recovered upon removal of cytochalasin. In conjunction with previous reports, the activity of cytochalasin on HSV entry suggests that a change in cytoskeletal structure following virus attachment triggers a microfilament activity important for internalization of HSV by HEp-2 cells.

Original languageEnglish (US)
Pages (from-to)1601-1605
Number of pages5
JournalJournal of General Virology
Volume66
Issue number7
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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