Inhibition of H+-ATPase-mediated proton pumping in Cryptococcus neoformans by a novel conjugated styryl ketone

Elias K. Manavathu, Jonathan R. Dimmock, Sarvesh C. Vashishtha, P. H. Chandrasekar

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We investigated the in vitro susceptibility of clinical isolates of Cryptococcus neoformans to the novel conjugated styryl ketone NC1175 by broth microdilution. The MIC90 and the MFC of NC1175 for C. neoformans were 1 and 2 mg/L, respectively. NC1175 at low concentrations (1-4 mg/L) completely inhibited the glucose-induced acidification of the external medium caused by the extrusion of intracellular protons mediated by the plasma membrane located H+-ATPase. These data suggest that NC1175 is a fungicidal agent for C. neoformans and its possible cellular target(s) include the H+-ATPase.

Original languageEnglish (US)
Pages (from-to)491-494
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume47
Issue number4
StatePublished - Apr 26 2001

ASJC Scopus subject areas

  • Pharmacology
  • Microbiology

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    Manavathu, E. K., Dimmock, J. R., Vashishtha, S. C., & Chandrasekar, P. H. (2001). Inhibition of H+-ATPase-mediated proton pumping in Cryptococcus neoformans by a novel conjugated styryl ketone. Journal of Antimicrobial Chemotherapy, 47(4), 491-494.