Inhibition of In Vitro Intracellular Growth of Trypanosoma cruzi by Dicationic Compounds

Edwin C. Rowland, Deborah Moore-Lai, John R. Seed, Chad E. Stephens, David W. Boykin

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Dicationic compounds, which are derivatives of pentamidine, are being developed for use as antiprotozoal drugs. These compounds bind to the minor groove of DNA and are thought to inhibit DNA-dependent enzymes and thereby prevent cellular replication by protozoans. The objective of this study was to test the ability of a group of these compounds to inhibit the intracellular and extracellular reproduction of Trypanosoma cruzi in vitro. At present, there are few drugs in use capable of inhibiting the intracellular stages of this parasite, and therefore compounds with this ability would be of value. Cultures of mouse fibroblasts were infected and treated with doses of dicationic compounds, and the numbers of parasites released at the end of the 5- to 7-day growth cycle were determined. Five of the compounds tested were found to be effective at inhibiting T. cruzi growth at doses that were not toxic to the host cells. The compound found most effective (DB709) inhibited parasite release at the low concentration of 0.8 ng/ml, justifying further study. These results suggest that dicationic compounds may have potential as chemotherapy against T. cruzi infection.

Original languageEnglish (US)
Pages (from-to)1078-1080
Number of pages3
JournalJournal of Parasitology
Volume89
Issue number5
DOIs
StatePublished - Oct 1 2003

Fingerprint

Trypanosoma cruzi
parasite
Parasites
parasites
drug
Growth
Pentamidine
chemotherapy
DNA
drugs
Poisons
dosage
Pharmaceutical Preparations
drug therapy
fibroblasts
Reproduction
Protozoa
Fibroblasts
enzyme
Drug Therapy

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Inhibition of In Vitro Intracellular Growth of Trypanosoma cruzi by Dicationic Compounds. / Rowland, Edwin C.; Moore-Lai, Deborah; Seed, John R.; Stephens, Chad E.; Boykin, David W.

In: Journal of Parasitology, Vol. 89, No. 5, 01.10.2003, p. 1078-1080.

Research output: Contribution to journalArticle

Rowland, Edwin C. ; Moore-Lai, Deborah ; Seed, John R. ; Stephens, Chad E. ; Boykin, David W. / Inhibition of In Vitro Intracellular Growth of Trypanosoma cruzi by Dicationic Compounds. In: Journal of Parasitology. 2003 ; Vol. 89, No. 5. pp. 1078-1080.
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