Inhibition of neuronal 5-HT uptake by ketamine, but not halothane, involves disruption of substrate recognition by the transporter

Dan C Martin, Robert P. Introna, Robert S. Aronstam

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

The effects of halothane and ketamine on (1) serotonin (5-hydroxytryptamine; 5-HT) uptake and (2) paroxetine binding to the 5-HT transporter in neuronal membranes were determined in rat brain. Both anesthetics inhibited the uptake of [3H]5-HT by synaptosomes, but only ketamine affected binding of [3H]paroxetine to the 5-HT transporter. Saturation analysis indicated that ketamine inhibition of [3H]paroxetine binding was competitive (Ki = 18.8 μM). These results indicate that halothane and ketamine inhibit 5-HT transport by different mechanisms.

Original languageEnglish (US)
Pages (from-to)99-103
Number of pages5
JournalNeuroscience Letters
Volume112
Issue number1
DOIs
StatePublished - Apr 20 1990

Keywords

  • 5-Hydroxytryptamine
  • Anesthetic
  • Brain
  • Halothane
  • Imipramine
  • Ketamine
  • Paroxetine
  • Serotonin
  • Serotonin receptor
  • Serotonin uptake

ASJC Scopus subject areas

  • Neuroscience(all)

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