Inhibition of rat and human prolidases by captopril

Vadivel Ganapathy, Steven J. Pashley, R. August Roesel, David Henry Pashley, Frederick H. Leibach

Research output: Contribution to journalArticle

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Abstract

We examined the effects of captopril on prolidase activity of crude homogenates of various tissues in the rat and in the human. It was found that captopril caused significant inhibition of prolidases from liver, kidney, and intestine in both species, whereas it showed minimal inhibitory effect on erythrocyte prolidase. In the rat, Ki for the inhibition of kidney and liver prolidases was in the range of 25-35 μM while, in the human, the value was considerably higher. The nature of inhibition was found to be competitive both in the rat and in the human. Oral administration of captopril (daily dose, 40 mg/kg) for 7 days in the rat resulted in increased urinary excretion of peptide-bound 4-hydroxy-l-proline compared to controls, indicating in vivo inhibition of tissue prolidases by captopril.

Original languageEnglish (US)
Pages (from-to)1287-1291
Number of pages5
JournalBiochemical Pharmacology
Volume34
Issue number8
DOIs
StatePublished - Apr 15 1985

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proline dipeptidase
Captopril
Rats
Liver
Hydroxyproline
Tissue
Kidney
Proline
Intestines
Oral Administration
Erythrocytes
Peptides

ASJC Scopus subject areas

  • Pharmacology

Cite this

Ganapathy, V., Pashley, S. J., August Roesel, R., Pashley, D. H., & Leibach, F. H. (1985). Inhibition of rat and human prolidases by captopril. Biochemical Pharmacology, 34(8), 1287-1291. https://doi.org/10.1016/0006-2952(85)90507-6

Inhibition of rat and human prolidases by captopril. / Ganapathy, Vadivel; Pashley, Steven J.; August Roesel, R.; Pashley, David Henry; Leibach, Frederick H.

In: Biochemical Pharmacology, Vol. 34, No. 8, 15.04.1985, p. 1287-1291.

Research output: Contribution to journalArticle

Ganapathy, V, Pashley, SJ, August Roesel, R, Pashley, DH & Leibach, FH 1985, 'Inhibition of rat and human prolidases by captopril', Biochemical Pharmacology, vol. 34, no. 8, pp. 1287-1291. https://doi.org/10.1016/0006-2952(85)90507-6
Ganapathy V, Pashley SJ, August Roesel R, Pashley DH, Leibach FH. Inhibition of rat and human prolidases by captopril. Biochemical Pharmacology. 1985 Apr 15;34(8):1287-1291. https://doi.org/10.1016/0006-2952(85)90507-6
Ganapathy, Vadivel ; Pashley, Steven J. ; August Roesel, R. ; Pashley, David Henry ; Leibach, Frederick H. / Inhibition of rat and human prolidases by captopril. In: Biochemical Pharmacology. 1985 ; Vol. 34, No. 8. pp. 1287-1291.
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