Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians

I. A. Gorshkova, B. A. Gorshkov, V. A. Stonik

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

I. A. Gorshkova, B. A. Gorshkov and V. A. Stonik. Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians. Toxicon 27, 927-936, 1989.-The effect of triterpene glycosides from holothurians on Na+-K+-ATPase of rat brain was investigated. The marine glycosides are irreversible inhibitors of the enzyme with an average I50 value of 10-4 M. ATP had a low protective effect against inhibition. The inhibitory effect was increased by preincubation with MgCl2. There was alteration of the activation curve of Na+-K+-ATPase by NaCl and KCl in the presence of glycosides. Triterpene glycosides inhibited the K+-phosphatase activity, but to a smaller degree than the ATPase activity. Na+-K+-ATPase of pig kidney was less sensitive to the marine triterpene glycosides than the brain enzyme. The marine glycosides did not alter the specific binding of [3H]-ouabain to the Na+-K+-ATPase.

Original languageEnglish (US)
Pages (from-to)927-936
Number of pages10
JournalToxicon
Volume27
Issue number8
DOIs
StatePublished - Jan 1 1989

Fingerprint

Triterpenes
Glycosides
Adenosine Triphosphatases
Rats
Brain
Magnesium Chloride
Enzyme Inhibitors
Ouabain
sodium-translocating ATPase
Phosphoric Monoester Hydrolases
Swine
Adenosine Triphosphate
Chemical activation
Kidney
Enzymes

ASJC Scopus subject areas

  • Toxicology

Cite this

Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians. / Gorshkova, I. A.; Gorshkov, B. A.; Stonik, V. A.

In: Toxicon, Vol. 27, No. 8, 01.01.1989, p. 927-936.

Research output: Contribution to journalArticle

Gorshkova, I. A. ; Gorshkov, B. A. ; Stonik, V. A. / Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians. In: Toxicon. 1989 ; Vol. 27, No. 8. pp. 927-936.
@article{58820f2971f74ad8bfd303384116853e,
title = "Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians",
abstract = "I. A. Gorshkova, B. A. Gorshkov and V. A. Stonik. Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians. Toxicon 27, 927-936, 1989.-The effect of triterpene glycosides from holothurians on Na+-K+-ATPase of rat brain was investigated. The marine glycosides are irreversible inhibitors of the enzyme with an average I50 value of 10-4 M. ATP had a low protective effect against inhibition. The inhibitory effect was increased by preincubation with MgCl2. There was alteration of the activation curve of Na+-K+-ATPase by NaCl and KCl in the presence of glycosides. Triterpene glycosides inhibited the K+-phosphatase activity, but to a smaller degree than the ATPase activity. Na+-K+-ATPase of pig kidney was less sensitive to the marine triterpene glycosides than the brain enzyme. The marine glycosides did not alter the specific binding of [3H]-ouabain to the Na+-K+-ATPase.",
author = "Gorshkova, {I. A.} and Gorshkov, {B. A.} and Stonik, {V. A.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1016/0041-0101(89)90104-9",
language = "English (US)",
volume = "27",
pages = "927--936",
journal = "Toxicon",
issn = "0041-0101",
publisher = "Elsevier Limited",
number = "8",

}

TY - JOUR

T1 - Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians

AU - Gorshkova, I. A.

AU - Gorshkov, B. A.

AU - Stonik, V. A.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - I. A. Gorshkova, B. A. Gorshkov and V. A. Stonik. Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians. Toxicon 27, 927-936, 1989.-The effect of triterpene glycosides from holothurians on Na+-K+-ATPase of rat brain was investigated. The marine glycosides are irreversible inhibitors of the enzyme with an average I50 value of 10-4 M. ATP had a low protective effect against inhibition. The inhibitory effect was increased by preincubation with MgCl2. There was alteration of the activation curve of Na+-K+-ATPase by NaCl and KCl in the presence of glycosides. Triterpene glycosides inhibited the K+-phosphatase activity, but to a smaller degree than the ATPase activity. Na+-K+-ATPase of pig kidney was less sensitive to the marine triterpene glycosides than the brain enzyme. The marine glycosides did not alter the specific binding of [3H]-ouabain to the Na+-K+-ATPase.

AB - I. A. Gorshkova, B. A. Gorshkov and V. A. Stonik. Inhibition of rat brain Na+-K+-ATPase by triterpene glycosides from holothurians. Toxicon 27, 927-936, 1989.-The effect of triterpene glycosides from holothurians on Na+-K+-ATPase of rat brain was investigated. The marine glycosides are irreversible inhibitors of the enzyme with an average I50 value of 10-4 M. ATP had a low protective effect against inhibition. The inhibitory effect was increased by preincubation with MgCl2. There was alteration of the activation curve of Na+-K+-ATPase by NaCl and KCl in the presence of glycosides. Triterpene glycosides inhibited the K+-phosphatase activity, but to a smaller degree than the ATPase activity. Na+-K+-ATPase of pig kidney was less sensitive to the marine triterpene glycosides than the brain enzyme. The marine glycosides did not alter the specific binding of [3H]-ouabain to the Na+-K+-ATPase.

UR - http://www.scopus.com/inward/record.url?scp=0024361055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024361055&partnerID=8YFLogxK

U2 - 10.1016/0041-0101(89)90104-9

DO - 10.1016/0041-0101(89)90104-9

M3 - Article

VL - 27

SP - 927

EP - 936

JO - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 8

ER -