Inhibition of release of tumor necrosis factor alpha (tnf-α) from human vascular tissue and smooth muscle cells by steroids

W. H. Newman, L. M. Zhang, S. K. Leeper-Woodford, J. H. Johnson, I. J. Shaker, S. K. Erceg, Manuel R Castresana

Research output: Contribution to journalArticle

Abstract

We found that bacterial lipopolysaccharide (LPS) stimulates release of TNF-α from human vascular tissue and smooth muscle cells (SMC). Here, we tested the hypothesis that release could be inhibited by pretreatment with corticosteroids. With IRB approval and patient consent, excess saphenous vein and internal mammary artery were obtained during coronary bypass. SMC were cultured from these vessels. Confluent SMC in 48 well culture dishes were exposed to 20 μg/ml LPS following pretreatment for 18 hr with 0.1, 1.0, and 10.0 μM dexamethasone (DEX). At 1, 3, 6, 18, and 24 hr the medium was removed and analyzed for biologically active TNF-α by the L929 cell cytotoxicity assay, SMC exposed to LPS but not treated with DEX served as controls. In control SMC, LPS stimulated TNF-α release in a time dependent manner from (indetectable levels at zero time to a peak of 18.0 ± 1.8 units/mg cell protein at 6 hr (n=8). DEX inhibited release at all time points. For instance at 6 hr, TNF-α was 5.6 ± 1.5, 4.6 ± 2.3 and 0.2 ± 0.2 units/mg for cells pretreated with 0.1, 1.0, and 10.0 μM DEX respectively (p < 0.05 vs. control). Additionally, time dependent LPS stimulated release of TNF-α was determined in segments of vascular tissue taken from 5 patients who received 1 g methlyprednisolone (MP) by i.v. injection during induction of anesthesia and compared to release from patients who had not received MP. In patients treated with MP, LPS did not stimulate release from tissues incubated for up to 24 hr. In contrast, LPS induced release of TNF-α from tissues of untreated patients (e.g., 733 ± 33 units/g tissue at 6 hr, n=9 ) These results confirm that human vascular tissue is a source of TNF-α and that release can be inhibited by corticosteriods.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996
Externally publishedYes

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Vascular Smooth Muscle
Smooth Muscle Myocytes
Lipopolysaccharides
Muscle
Tumor Necrosis Factor-alpha
Steroids
Cells
Tissue
Dexamethasone
Blood Vessels
Mammary Arteries
Research Ethics Committees
Saphenous Vein
Cytotoxicity
Assays
Adrenal Cortex Hormones
Anesthesia
Injections
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Newman, W. H., Zhang, L. M., Leeper-Woodford, S. K., Johnson, J. H., Shaker, I. J., Erceg, S. K., & Castresana, M. R. (1996). Inhibition of release of tumor necrosis factor alpha (tnf-α) from human vascular tissue and smooth muscle cells by steroids. FASEB Journal, 10(3).

Inhibition of release of tumor necrosis factor alpha (tnf-α) from human vascular tissue and smooth muscle cells by steroids. / Newman, W. H.; Zhang, L. M.; Leeper-Woodford, S. K.; Johnson, J. H.; Shaker, I. J.; Erceg, S. K.; Castresana, Manuel R.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

Newman, WH, Zhang, LM, Leeper-Woodford, SK, Johnson, JH, Shaker, IJ, Erceg, SK & Castresana, MR 1996, 'Inhibition of release of tumor necrosis factor alpha (tnf-α) from human vascular tissue and smooth muscle cells by steroids', FASEB Journal, vol. 10, no. 3.
Newman WH, Zhang LM, Leeper-Woodford SK, Johnson JH, Shaker IJ, Erceg SK et al. Inhibition of release of tumor necrosis factor alpha (tnf-α) from human vascular tissue and smooth muscle cells by steroids. FASEB Journal. 1996 Dec 1;10(3).
Newman, W. H. ; Zhang, L. M. ; Leeper-Woodford, S. K. ; Johnson, J. H. ; Shaker, I. J. ; Erceg, S. K. ; Castresana, Manuel R. / Inhibition of release of tumor necrosis factor alpha (tnf-α) from human vascular tissue and smooth muscle cells by steroids. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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