Inhibition of reporter gene and Iridovirus-tiger frog virus in fish cell by RNA interference

Junfeng Xie, Ling Lü, Min Deng, Shaoping Weng, Jingyi Zhu, Yue Wu, Lin Gan, Siu Ming Chan, Jianguo He

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

We describe the specific silencing of reporter gene lacZ in FHM cells (muscle cells of fathead minnow, a fish cell line) by either expressing small hairpin RNAs (shRNAs) from plasmids or transfecting small interfering RNAs (siRNAs) transcribed in vitro. Two types of dsRNAs could inhibit reporter gene expression, and siRNAs were more effective, while both of them worked very well in HeLa cells. siRNAs were tested for silencing expression of the major capsid protein (MCP) encoded by tiger frog virus (TFV), an iridovirus causing severe disease in fish. siRNAs targeting mcp gene effectively inhibited TFV replication in fish cells as demonstrated by reduced mcp RNA level, postponed emergence of cytopathogenic effect, as well as reduced TFV titer and particles in cells. The results suggest that the siRNA method suppressed TFV efficiently in fish cells, providing a potential approach to the therapy of aquaculture viral diseases.

Original languageEnglish (US)
Pages (from-to)43-52
Number of pages10
JournalVirology
Volume338
Issue number1
DOIs
StatePublished - Jul 20 2005

Keywords

  • RNA interference
  • TFV
  • shRNA
  • siRNA

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Inhibition of reporter gene and Iridovirus-tiger frog virus in fish cell by RNA interference'. Together they form a unique fingerprint.

  • Cite this

    Xie, J., Lü, L., Deng, M., Weng, S., Zhu, J., Wu, Y., Gan, L., Chan, S. M., & He, J. (2005). Inhibition of reporter gene and Iridovirus-tiger frog virus in fish cell by RNA interference. Virology, 338(1), 43-52. https://doi.org/10.1016/j.virol.2005.04.040