Inhibition of suppressor cell function by cimetidine in a murine model

Zaiwang Jin, Ashir Kumar, Ronald P. Cleveland, Dennis L Murray, Donald B. Kaufman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Immunomodulatory effects of cimetidine, an H 2 histamine receptor antagonist, have been reported in humans and animals. To define these effects more clearly, the action of cimetidine on suppressor cell function was studied utilizing a murine model of contact hypersensitivity. Intravenous inoculation of BALB c mice with DNP-coupled syngeneic spleen cells induced the production of DNP-specific suppressor cells which could easily be demonstrated by a reduction in ear swelling after contact sensitization with 1-fluoro-2,4-dinitrobenzene (DNFB) following transfer of spleen and lymph node cells to naive syngeneic recipients. Cimetidine treatment of animals in which suppressor cells were induced resulted in an inability of these mice to transfer cellular suppression as measured by development of a normal immunologic response in the recipient mice. The effect of cimetidine was both dose and time related. While all groups receiving cimetidine showed some loss of suppressor cell function, the maximum effect (up to 100% inhibition) was seen when 50 mg/kg of cimetidine was administered intraperitoneally 2 days before or on the day of suppressor cell induction. Some restoration also occurred when cimetidine was given after the day of induction. It has been shown that suppressor cells possess histamine receptors which may be involved in suppressor cell activation. The results indicate that cimetidine may inhibit the functioning of these receptors.

Original languageEnglish (US)
Pages (from-to)350-356
Number of pages7
JournalClinical Immunology and Immunopathology
Volume38
Issue number3
DOIs
StatePublished - Jan 1 1986

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Cimetidine
Dinitrofluorobenzene
Histamine Receptors
Spleen
Histamine Antagonists
Contact Dermatitis
Ear
Lymph Nodes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

Cite this

Inhibition of suppressor cell function by cimetidine in a murine model. / Jin, Zaiwang; Kumar, Ashir; Cleveland, Ronald P.; Murray, Dennis L; Kaufman, Donald B.

In: Clinical Immunology and Immunopathology, Vol. 38, No. 3, 01.01.1986, p. 350-356.

Research output: Contribution to journalArticle

Jin, Zaiwang ; Kumar, Ashir ; Cleveland, Ronald P. ; Murray, Dennis L ; Kaufman, Donald B. / Inhibition of suppressor cell function by cimetidine in a murine model. In: Clinical Immunology and Immunopathology. 1986 ; Vol. 38, No. 3. pp. 350-356.
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