Inhibition of testicular and Vipera russelli snake venom hyaluronidase activity by Butea monosperma (Lam) Kuntze stem bark

Shaista Tarannum, Riyaz Mohamed, Bannikuppe S. Vishwanath

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

This study was carried out to investigate the anti-fertility and anti-venom activities of the extract of the stem bark of Butea monosperma by inhibiting hyaluronidase, which is a spreading factor and plays a role in fertilisation. Among ethanol, methanol and water extracts, the ethanol extract dose-dependently inhibited the ovine, mouse testicular and Vipera russelli snake venom hyaluronidase enzyme activities, with IC50 values 12.00±0.45, 49.40±1.58μg and 125.42±2.82μgmL-1, respectively. In a zymogram assay, the extract showed differential inhibition towards hyaluronidase isoform preferentially with low-molecular weight isoforms. The V. russelli snake venom-induced hemorrhage was significantly reduced at 1:05 ratio of venom-to-extract in mouse. The high antioxidant activity and total phenolic content in the ethanolic extract strongly correlated with the hyaluronidase inhibition. The above results justify the traditional use of the stem bark of B. monosperma as a contraceptive and a strong antidote to snake venom.

Original languageEnglish (US)
Pages (from-to)1708-1711
Number of pages4
JournalNatural Product Research
Volume26
Issue number18
DOIs
StatePublished - Sep 1 2012
Externally publishedYes

Keywords

  • anti-fertility
  • anti-snake venom
  • Butea monosperma
  • haemorrhage
  • hyaluronidase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Plant Science
  • Organic Chemistry

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