Inhibition of the H+/peptide cotransporter in the human intestinal cell line Caco-2 by cyclic AMP

Ulrike Muller, Matthias Brandsch, Puttur D. Prasad, You Jun Fei, Vadivel Ganapathy, Frederick H. Leibach

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Treatment of Caco-2 cells with cholera toxin inhibits the activity of the H+/peptide cotransporter. The effect of cholera toxin is mimicked by E. coli heat-labile enterotoxin, forskolin and isobutylmethylxanthine and is associated with an increase in cAMP levels in the cells. The inhibition is due to a decrease in the maximal velocity of the transport system. Inhibitors of protein kinase A and protein kinase C block the effect of cholera toxin. Interestingly, the H+/peptide cotransporter in Caco-2 cells does not possess any putative site for phosphorylation by protein kinase A but does possess sites for phosphorylation by protein kinase C. It appears that the cAMP-dependent inhibition of the H+/peptide cotransporter in Caco-2 cells is mediated through activation Of protein kinase C.

Original languageEnglish (US)
Pages (from-to)461-465
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number2
DOIs
StatePublished - Jan 17 1996

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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