Abstract
Treatment of Caco-2 cells with cholera toxin inhibits the activity of the H+/peptide cotransporter. The effect of cholera toxin is mimicked by E. coli heat-labile enterotoxin, forskolin and isobutylmethylxanthine and is associated with an increase in cAMP levels in the cells. The inhibition is due to a decrease in the maximal velocity of the transport system. Inhibitors of protein kinase A and protein kinase C block the effect of cholera toxin. Interestingly, the H+/peptide cotransporter in Caco-2 cells does not possess any putative site for phosphorylation by protein kinase A but does possess sites for phosphorylation by protein kinase C. It appears that the cAMP-dependent inhibition of the H+/peptide cotransporter in Caco-2 cells is mediated through activation Of protein kinase C.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 461-465 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 218 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 17 1996 |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
Inhibition of the H+/peptide cotransporter in the human intestinal cell line Caco-2 by cyclic AMP. / Muller, Ulrike; Brandsch, Matthias; Prasad, Puttur D; Fei, You Jun; Ganapathy, Vadivel; Leibach, Frederick H.
In: Biochemical and Biophysical Research Communications, Vol. 218, No. 2, 17.01.1996, p. 461-465.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of the H+/peptide cotransporter in the human intestinal cell line Caco-2 by cyclic AMP
AU - Muller, Ulrike
AU - Brandsch, Matthias
AU - Prasad, Puttur D
AU - Fei, You Jun
AU - Ganapathy, Vadivel
AU - Leibach, Frederick H.
PY - 1996/1/17
Y1 - 1996/1/17
N2 - Treatment of Caco-2 cells with cholera toxin inhibits the activity of the H+/peptide cotransporter. The effect of cholera toxin is mimicked by E. coli heat-labile enterotoxin, forskolin and isobutylmethylxanthine and is associated with an increase in cAMP levels in the cells. The inhibition is due to a decrease in the maximal velocity of the transport system. Inhibitors of protein kinase A and protein kinase C block the effect of cholera toxin. Interestingly, the H+/peptide cotransporter in Caco-2 cells does not possess any putative site for phosphorylation by protein kinase A but does possess sites for phosphorylation by protein kinase C. It appears that the cAMP-dependent inhibition of the H+/peptide cotransporter in Caco-2 cells is mediated through activation Of protein kinase C.
AB - Treatment of Caco-2 cells with cholera toxin inhibits the activity of the H+/peptide cotransporter. The effect of cholera toxin is mimicked by E. coli heat-labile enterotoxin, forskolin and isobutylmethylxanthine and is associated with an increase in cAMP levels in the cells. The inhibition is due to a decrease in the maximal velocity of the transport system. Inhibitors of protein kinase A and protein kinase C block the effect of cholera toxin. Interestingly, the H+/peptide cotransporter in Caco-2 cells does not possess any putative site for phosphorylation by protein kinase A but does possess sites for phosphorylation by protein kinase C. It appears that the cAMP-dependent inhibition of the H+/peptide cotransporter in Caco-2 cells is mediated through activation Of protein kinase C.
UR - http://www.scopus.com/inward/record.url?scp=0030070069&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030070069&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1996.0082
DO - 10.1006/bbrc.1996.0082
M3 - Article
VL - 218
SP - 461
EP - 465
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -