Inhibition of the H+/peptide cotransporter in the human intestinal cell line Caco-2 by cyclic AMP

Ulrike Muller, Matthias Brandsch, Puttur D Prasad, You Jun Fei, Vadivel Ganapathy, Frederick H. Leibach

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Treatment of Caco-2 cells with cholera toxin inhibits the activity of the H+/peptide cotransporter. The effect of cholera toxin is mimicked by E. coli heat-labile enterotoxin, forskolin and isobutylmethylxanthine and is associated with an increase in cAMP levels in the cells. The inhibition is due to a decrease in the maximal velocity of the transport system. Inhibitors of protein kinase A and protein kinase C block the effect of cholera toxin. Interestingly, the H+/peptide cotransporter in Caco-2 cells does not possess any putative site for phosphorylation by protein kinase A but does possess sites for phosphorylation by protein kinase C. It appears that the cAMP-dependent inhibition of the H+/peptide cotransporter in Caco-2 cells is mediated through activation Of protein kinase C.

Original languageEnglish (US)
Pages (from-to)461-465
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume218
Issue number2
DOIs
StatePublished - Jan 17 1996

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Caco-2 Cells
Cholera Toxin
Cyclic AMP
Protein Kinase C
Phosphorylation
Cells
Cyclic AMP-Dependent Protein Kinases
Cell Line
Peptides
Colforsin
Chemical activation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Inhibition of the H+/peptide cotransporter in the human intestinal cell line Caco-2 by cyclic AMP. / Muller, Ulrike; Brandsch, Matthias; Prasad, Puttur D; Fei, You Jun; Ganapathy, Vadivel; Leibach, Frederick H.

In: Biochemical and Biophysical Research Communications, Vol. 218, No. 2, 17.01.1996, p. 461-465.

Research output: Contribution to journalArticle

Muller, Ulrike ; Brandsch, Matthias ; Prasad, Puttur D ; Fei, You Jun ; Ganapathy, Vadivel ; Leibach, Frederick H. / Inhibition of the H+/peptide cotransporter in the human intestinal cell line Caco-2 by cyclic AMP. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 218, No. 2. pp. 461-465.
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