Inhibition of yeast-to-mycelium conversion of Candida albicans by conjugated styryl ketones

Elias Kurian Manavathu, Carri Duncan, Quallyna Porte, Muthukumaran Gunasekaran

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Candida albicans is a dimorphic pathogenic yeast capable of producing alternate morphological forms (yeast or mycelium) in response to environmental changes. The dimorphism of C. albicans plays an important role in the pathophysiology of this organism. The intracellular level of glutathione, which helps to maintain the oxidationreduction potential of the cell, is decreased significantly during the yeast-to-mycelium conversion implicating the possible involvement of thiols in the yeast-to-mycelium transition. To evaluate the possible participation of sulphydryl group(s) containing component(s) in the yeast-to-mycelium transition of C. albicans, we examined the effect of a group of newly synthesized thiol-alkylators on the production of germ tubes from yeast cells. Several conjugated styryl ketones which are thiol-alkylators, and p-chloromercuriphenylsulphonate (a known non-penetrating thiol-blocker) inhibited the yeast-to-mycelium conversion of C. albicans. The thiol-alkylators at 20 μM failed to inhibit four key enzymes (γ-glutamyltranspeptidase, glutathione reductase, glutathione S-transferase and glutathione peroxidase) involved in glutathione utilization indicating that the inhibition of yeast-to-mycelium conversion is not mediated by the inhibition of glutathione metabolic enzymes. Moreover, these results suggest that a key thiol-blocker sensitive component(s) containing a critical sulphydryl group(s) is involved in the yeast-tomycelium transition of C. albicans.

Original languageEnglish (US)
Pages (from-to)79-83
Number of pages5
JournalMycopathologia
Volume135
Issue number2
DOIs
StatePublished - Aug 1 1996

Fingerprint

Mycelium
ketones
Ketones
Candida albicans
mycelium
Yeasts
yeasts
thiols
Sulfhydryl Compounds
Alkylating Agents
Glutathione
glutathione
4-Chloromercuribenzenesulfonate
group effect
Glutathione Reductase
germ tube
Enzymes
Glutathione Peroxidase
enzymes
pathophysiology

Keywords

  • Candida albicans
  • dimorphism
  • glutathione
  • styryl ketones
  • thiol-blockers

ASJC Scopus subject areas

  • veterinary (miscalleneous)
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Plant Science
  • Agronomy and Crop Science
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Inhibition of yeast-to-mycelium conversion of Candida albicans by conjugated styryl ketones. / Manavathu, Elias Kurian; Duncan, Carri; Porte, Quallyna; Gunasekaran, Muthukumaran.

In: Mycopathologia, Vol. 135, No. 2, 01.08.1996, p. 79-83.

Research output: Contribution to journalArticle

Manavathu, Elias Kurian ; Duncan, Carri ; Porte, Quallyna ; Gunasekaran, Muthukumaran. / Inhibition of yeast-to-mycelium conversion of Candida albicans by conjugated styryl ketones. In: Mycopathologia. 1996 ; Vol. 135, No. 2. pp. 79-83.
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