Initial characterization of whole-cell currents from freshly dissociated corneal keratocytes

Mitchell A. Watsky, James L. Rae

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The perforated patch technique was utilized to obtain whole-cell currents from freshly dissociated rabbit corneal keratocytes. We describe and provide the initial characterization of two distinct whole cell currents in rabbit keratocytes: a K+-selective delayed rectifier and a voltage-sensitive, tetrodotoxin blockable Na+ current. The voltage-sensitive Na+ current is of sufficient magnitude to allow us to initiate action potentials when current-clamping the cells. This is the first detailed electrophysiological study of corneal keratocytes.

Original languageEnglish (US)
Pages (from-to)127-134
Number of pages8
JournalCurrent Eye Research
Volume11
Issue number2
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

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ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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