TY - JOUR
T1 - Initial In Vitro Biological Response to Contemporary Endodontic Sealers
AU - Bouillaguet, Serge
AU - Wataha, John C.
AU - Tay, Franklin Chi Meng
AU - Brackett, Martha G
AU - Lockwood, Petra E.
PY - 2006/10/1
Y1 - 2006/10/1
N2 - The objective of this study was to evaluate the cytotoxicity of three endodontic sealers (AH Plus/Maillefer-Dentsply, Epiphany/Pentron, GuttaFlow, Coltene-Whaledent). Materials were mixed according to the manufacturer instructions and packed into Teflon molds (10 × 1 mm). For cytotoxicity testing (MTT method), the specimens were placed in contact with cultured cells, then evaluated at two subsequent time points (24 or 72 h). In addition to testing the mixed materials, 5 μl of primer liquid (GuttaFlow and Epiphany) and resin solvents (HEMA, ethanol, sterile water, or acetone) were added directly in culture for 24 and 72 h. The results showed that most materials pose significant cytotoxic risks and that cytotoxicity generally increased with time. At 72 h, GuttaFlow became significantly less toxic than AH Plus, Epiphany sealer, and Resilon. The current results support the need to continue to develop better endodontic sealers that combine the excellent sealing and bonding properties of resins with acceptable biological properties for endodontic applications.
AB - The objective of this study was to evaluate the cytotoxicity of three endodontic sealers (AH Plus/Maillefer-Dentsply, Epiphany/Pentron, GuttaFlow, Coltene-Whaledent). Materials were mixed according to the manufacturer instructions and packed into Teflon molds (10 × 1 mm). For cytotoxicity testing (MTT method), the specimens were placed in contact with cultured cells, then evaluated at two subsequent time points (24 or 72 h). In addition to testing the mixed materials, 5 μl of primer liquid (GuttaFlow and Epiphany) and resin solvents (HEMA, ethanol, sterile water, or acetone) were added directly in culture for 24 and 72 h. The results showed that most materials pose significant cytotoxic risks and that cytotoxicity generally increased with time. At 72 h, GuttaFlow became significantly less toxic than AH Plus, Epiphany sealer, and Resilon. The current results support the need to continue to develop better endodontic sealers that combine the excellent sealing and bonding properties of resins with acceptable biological properties for endodontic applications.
KW - Biocompatibility
KW - MTT
KW - cell-culture
KW - endodontics
KW - resilon
UR - http://www.scopus.com/inward/record.url?scp=33748579626&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748579626&partnerID=8YFLogxK
U2 - 10.1016/j.joen.2006.05.006
DO - 10.1016/j.joen.2006.05.006
M3 - Article
C2 - 16982280
AN - SCOPUS:33748579626
SN - 0099-2399
VL - 32
SP - 989
EP - 992
JO - Journal of Endodontics
JF - Journal of Endodontics
IS - 10
ER -