Initial In Vitro Biological Response to Contemporary Endodontic Sealers

Serge Bouillaguet, John C. Wataha, Franklin Chi Meng Tay, Martha G Brackett, Petra E. Lockwood

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The objective of this study was to evaluate the cytotoxicity of three endodontic sealers (AH Plus/Maillefer-Dentsply, Epiphany/Pentron, GuttaFlow, Coltene-Whaledent). Materials were mixed according to the manufacturer instructions and packed into Teflon molds (10 × 1 mm). For cytotoxicity testing (MTT method), the specimens were placed in contact with cultured cells, then evaluated at two subsequent time points (24 or 72 h). In addition to testing the mixed materials, 5 μl of primer liquid (GuttaFlow and Epiphany) and resin solvents (HEMA, ethanol, sterile water, or acetone) were added directly in culture for 24 and 72 h. The results showed that most materials pose significant cytotoxic risks and that cytotoxicity generally increased with time. At 72 h, GuttaFlow became significantly less toxic than AH Plus, Epiphany sealer, and Resilon. The current results support the need to continue to develop better endodontic sealers that combine the excellent sealing and bonding properties of resins with acceptable biological properties for endodontic applications.

Original languageEnglish (US)
Pages (from-to)989-992
Number of pages4
JournalJournal of Endodontics
Volume32
Issue number10
DOIs
StatePublished - Oct 1 2006

Fingerprint

Endodontics
Materials Testing
Poisons
Polytetrafluoroethylene
Acetone
Cultured Cells
Fungi
Ethanol
Water
GuttaFlow
In Vitro Techniques
epoxy resin-based root canal sealer

Keywords

  • Biocompatibility
  • MTT
  • cell-culture
  • endodontics
  • resilon

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Initial In Vitro Biological Response to Contemporary Endodontic Sealers. / Bouillaguet, Serge; Wataha, John C.; Tay, Franklin Chi Meng; Brackett, Martha G; Lockwood, Petra E.

In: Journal of Endodontics, Vol. 32, No. 10, 01.10.2006, p. 989-992.

Research output: Contribution to journalArticle

Bouillaguet, Serge ; Wataha, John C. ; Tay, Franklin Chi Meng ; Brackett, Martha G ; Lockwood, Petra E. / Initial In Vitro Biological Response to Contemporary Endodontic Sealers. In: Journal of Endodontics. 2006 ; Vol. 32, No. 10. pp. 989-992.
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