Initiation and processing in vitro of the primary translation products of guinea-pig caseins.

R. K. Craig, P. A. Perera, A. Mellor, A. E. Smith

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

1. Guinea-pig caseins synthesized in a mRNA-directed wheat-germ cell-free protein-synthesizing system represent the primary translation products, even though they appear to be of lower molecular weight when analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis in parallel with caseins isolated from guinea-pig milk. 2. Identification of the N-terminal dipeptide of the primary translational product of caseins A, B and C and alpha-lactalbumin showed that all shared a common sequence, which was identified as either Met-Arg or Met-Lys. 3. Procedures utilizing methionyl-tRNAfMet or methionyl-tRNAMet in the presence or absence of microsomal membranes during translation provide a rapid method of distinguishing between N-terminal processing of peptides synthesized in vitro and other post-translational modifications (glycosylation, phosphorylation), which also result in a change in mobility of peptides when analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. 4. The results demonstrate that guinea-pig caseins, in common with most other secretory proteins, are synthesized with transient N-terminal 'signal'-peptide extensions, which are cleaved during synthesis in the presence of microsomal membranes.

Original languageEnglish (US)
Pages (from-to)261-267
Number of pages7
JournalThe Biochemical journal
Volume184
Issue number2
DOIs
StatePublished - Jan 1 1979

Fingerprint

Caseins
Guinea Pigs
Lactalbumin
Processing
Electrophoresis
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
RNA, Transfer, Met
Glycosylation
Membranes
Peptides
Phosphorylation
Dipeptides
Post Translational Protein Processing
Protein Sorting Signals
Germ Cells
Triticum
Milk
Proteins
Molecular Weight

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Initiation and processing in vitro of the primary translation products of guinea-pig caseins. / Craig, R. K.; Perera, P. A.; Mellor, A.; Smith, A. E.

In: The Biochemical journal, Vol. 184, No. 2, 01.01.1979, p. 261-267.

Research output: Contribution to journalArticle

Craig, R. K. ; Perera, P. A. ; Mellor, A. ; Smith, A. E. / Initiation and processing in vitro of the primary translation products of guinea-pig caseins. In: The Biochemical journal. 1979 ; Vol. 184, No. 2. pp. 261-267.
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