Injury-elicited differential transcriptional regulation of phospholipid growth factor receptors in the cornea

De An Wang, Haiming Du, Jonathan H. Jaggar, David N. Brindley, Gabor J. Tigyi, Mitchell Aaron Watsky

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The phospholipid growth factors (PLGFs), including lysophosphatidic acid (LPA), have been implicated in corneal wound healing. PLGF concentrations and activities are elevated after corneal injury. Using real-time PCR, we quantified receptor mRNA levels in the healing rabbit cornea. In intact corneas, transcripts for S1P1, LPA1, and LPA3 receptor subtypes were detected, as was lipid phosphate phosphatase 1 (LPP1). After wounding, the trend for endothelium and keratocytes was for significant decreases in transcript numbers for the three receptor subtypes, whereas epithelial cells showed increased transcript numbers, except for an S1P1 decrease in healing cells. LPP1 transcript numbers were decreased in keratocytes and endothelium, although LPP-specific activity was unchanged. LPA-elicited Ca2+ transients were significantly reduced in the healing endothelium. Consistent with reduced LPA3 receptor numbers, dioctylglycerol pyrophosphate, a selective antagonist, reduced LPA-induced Ca2+ transients 2.7-fold in nonwounded epithelium but only 1.5-fold in wound-healing endothelium. These data for the first time establish physiologically relevant differential changes in the expression of PLGF receptor subtypes and provide evidence for the changing role of LPA3 receptors in endothelial cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume283
Issue number6 52-6
StatePublished - Dec 1 2002
Externally publishedYes

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Lysophosphatidic Acid Receptors
Growth Factor Receptors
Cornea
Endothelium
Phospholipids
Wounds and Injuries
Wound Healing
Intercellular Signaling Peptides and Proteins
Lysosphingolipid Receptors
Real-Time Polymerase Chain Reaction
Epithelium
Endothelial Cells
Epithelial Cells
Rabbits
Messenger RNA
lysophosphatidic acid

Keywords

  • Calcium
  • Diacylglycerol pyrophosphate
  • Lysophosphatidic acid
  • Phospholipid
  • Wound healing

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Injury-elicited differential transcriptional regulation of phospholipid growth factor receptors in the cornea. / Wang, De An; Du, Haiming; Jaggar, Jonathan H.; Brindley, David N.; Tigyi, Gabor J.; Watsky, Mitchell Aaron.

In: American Journal of Physiology - Cell Physiology, Vol. 283, No. 6 52-6, 01.12.2002.

Research output: Contribution to journalArticle

Wang, De An ; Du, Haiming ; Jaggar, Jonathan H. ; Brindley, David N. ; Tigyi, Gabor J. ; Watsky, Mitchell Aaron. / Injury-elicited differential transcriptional regulation of phospholipid growth factor receptors in the cornea. In: American Journal of Physiology - Cell Physiology. 2002 ; Vol. 283, No. 6 52-6.
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