Innate lymphoid cells: a paradigm for low SSI in cleft lip repair

Erika Simmerman, Xu Qin, Brendan Marshall, Libby Perry, Lei Cai, Tailing Wang, Jack C Yu, Omid Akbari, Babak Baban

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background Cleft lip and palate reconstructions demonstrate significantly lower surgical site infection rates compared with clean-contaminated cases, prompting investigation into the pathophysiology causing this discrepancy. Recent studies have identified a new group of innate lymphocytes called innate lymphoid cells (ILCs), located in barrier surfaces of the skin, airways, and intestine. Our objectives were to explore for the first time the presence of ILCs in the vermillion of neonates and young children undergoing cleft lip reconstruction and characterize their composition by measuring the three classes of ILCs. Materials and methods Lip tissue samples were collected from 13 subjects undergoing vermillion resection during cleft lip reconstructive surgery. Preparative, transmission electron microscopy, and analytical flow cytometry were performed. The functionality of ILCs was tested in terms of their capacity to produce type 1 (IFN-γ/TNF-α), type 2 (IL-5/IL-13), and type 3 (IL-17/IL-22) cytokines. Data were analyzed using Student t test or the analysis of variance to establish significance (P < 0.05) among groups for all other data. Results All three classes of ILCs were detected and visualized in the tissue samples. In all samples, the level of ILC2 subset was significantly higher than the other two ILC subsets (P < 0.01), followed by the ILC1 subset, which was present in significantly higher levels than the ILC3 subset (P < 0.05). Conclusions Our data place ILCs for the first time in the interface of oral mucosal immunity, tissue microenvironment, and homeostasis during and after tissue development, possibly explaining lower infection rates in cleft lip or palate reconstructions.

Original languageEnglish (US)
Pages (from-to)312-317
Number of pages6
JournalJournal of Surgical Research
Volume205
Issue number2
DOIs
StatePublished - Oct 1 2016

Fingerprint

Lip
Lymphocytes
Cleft Lip
Cleft Palate
Reconstructive Surgical Procedures
Surgical Wound Infection
Mucosal Immunity
Interleukin-13
Interleukin-17
Interleukin-3
Interleukin-5
Transmission Electron Microscopy
Intestines
Analysis of Variance
Flow Cytometry
Mucous Membrane
Homeostasis
Newborn Infant
Cytokines
Students

Keywords

  • Cleft lip reconstruction
  • Cleft palate reconstruction
  • Innate immunity
  • Innate lymphoid cells
  • Oral mucosa immunity
  • Oral tissue microenvironment

ASJC Scopus subject areas

  • Surgery

Cite this

Innate lymphoid cells : a paradigm for low SSI in cleft lip repair. / Simmerman, Erika; Qin, Xu; Marshall, Brendan; Perry, Libby; Cai, Lei; Wang, Tailing; Yu, Jack C; Akbari, Omid; Baban, Babak.

In: Journal of Surgical Research, Vol. 205, No. 2, 01.10.2016, p. 312-317.

Research output: Contribution to journalArticle

Simmerman, E, Qin, X, Marshall, B, Perry, L, Cai, L, Wang, T, Yu, JC, Akbari, O & Baban, B 2016, 'Innate lymphoid cells: a paradigm for low SSI in cleft lip repair', Journal of Surgical Research, vol. 205, no. 2, pp. 312-317. https://doi.org/10.1016/j.jss.2016.06.081
Simmerman E, Qin X, Marshall B, Perry L, Cai L, Wang T et al. Innate lymphoid cells: a paradigm for low SSI in cleft lip repair. Journal of Surgical Research. 2016 Oct 1;205(2):312-317. https://doi.org/10.1016/j.jss.2016.06.081
Simmerman, Erika ; Qin, Xu ; Marshall, Brendan ; Perry, Libby ; Cai, Lei ; Wang, Tailing ; Yu, Jack C ; Akbari, Omid ; Baban, Babak. / Innate lymphoid cells : a paradigm for low SSI in cleft lip repair. In: Journal of Surgical Research. 2016 ; Vol. 205, No. 2. pp. 312-317.
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abstract = "Background Cleft lip and palate reconstructions demonstrate significantly lower surgical site infection rates compared with clean-contaminated cases, prompting investigation into the pathophysiology causing this discrepancy. Recent studies have identified a new group of innate lymphocytes called innate lymphoid cells (ILCs), located in barrier surfaces of the skin, airways, and intestine. Our objectives were to explore for the first time the presence of ILCs in the vermillion of neonates and young children undergoing cleft lip reconstruction and characterize their composition by measuring the three classes of ILCs. Materials and methods Lip tissue samples were collected from 13 subjects undergoing vermillion resection during cleft lip reconstructive surgery. Preparative, transmission electron microscopy, and analytical flow cytometry were performed. The functionality of ILCs was tested in terms of their capacity to produce type 1 (IFN-γ/TNF-α), type 2 (IL-5/IL-13), and type 3 (IL-17/IL-22) cytokines. Data were analyzed using Student t test or the analysis of variance to establish significance (P < 0.05) among groups for all other data. Results All three classes of ILCs were detected and visualized in the tissue samples. In all samples, the level of ILC2 subset was significantly higher than the other two ILC subsets (P < 0.01), followed by the ILC1 subset, which was present in significantly higher levels than the ILC3 subset (P < 0.05). Conclusions Our data place ILCs for the first time in the interface of oral mucosal immunity, tissue microenvironment, and homeostasis during and after tissue development, possibly explaining lower infection rates in cleft lip or palate reconstructions.",
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N2 - Background Cleft lip and palate reconstructions demonstrate significantly lower surgical site infection rates compared with clean-contaminated cases, prompting investigation into the pathophysiology causing this discrepancy. Recent studies have identified a new group of innate lymphocytes called innate lymphoid cells (ILCs), located in barrier surfaces of the skin, airways, and intestine. Our objectives were to explore for the first time the presence of ILCs in the vermillion of neonates and young children undergoing cleft lip reconstruction and characterize their composition by measuring the three classes of ILCs. Materials and methods Lip tissue samples were collected from 13 subjects undergoing vermillion resection during cleft lip reconstructive surgery. Preparative, transmission electron microscopy, and analytical flow cytometry were performed. The functionality of ILCs was tested in terms of their capacity to produce type 1 (IFN-γ/TNF-α), type 2 (IL-5/IL-13), and type 3 (IL-17/IL-22) cytokines. Data were analyzed using Student t test or the analysis of variance to establish significance (P < 0.05) among groups for all other data. Results All three classes of ILCs were detected and visualized in the tissue samples. In all samples, the level of ILC2 subset was significantly higher than the other two ILC subsets (P < 0.01), followed by the ILC1 subset, which was present in significantly higher levels than the ILC3 subset (P < 0.05). Conclusions Our data place ILCs for the first time in the interface of oral mucosal immunity, tissue microenvironment, and homeostasis during and after tissue development, possibly explaining lower infection rates in cleft lip or palate reconstructions.

AB - Background Cleft lip and palate reconstructions demonstrate significantly lower surgical site infection rates compared with clean-contaminated cases, prompting investigation into the pathophysiology causing this discrepancy. Recent studies have identified a new group of innate lymphocytes called innate lymphoid cells (ILCs), located in barrier surfaces of the skin, airways, and intestine. Our objectives were to explore for the first time the presence of ILCs in the vermillion of neonates and young children undergoing cleft lip reconstruction and characterize their composition by measuring the three classes of ILCs. Materials and methods Lip tissue samples were collected from 13 subjects undergoing vermillion resection during cleft lip reconstructive surgery. Preparative, transmission electron microscopy, and analytical flow cytometry were performed. The functionality of ILCs was tested in terms of their capacity to produce type 1 (IFN-γ/TNF-α), type 2 (IL-5/IL-13), and type 3 (IL-17/IL-22) cytokines. Data were analyzed using Student t test or the analysis of variance to establish significance (P < 0.05) among groups for all other data. Results All three classes of ILCs were detected and visualized in the tissue samples. In all samples, the level of ILC2 subset was significantly higher than the other two ILC subsets (P < 0.01), followed by the ILC1 subset, which was present in significantly higher levels than the ILC3 subset (P < 0.05). Conclusions Our data place ILCs for the first time in the interface of oral mucosal immunity, tissue microenvironment, and homeostasis during and after tissue development, possibly explaining lower infection rates in cleft lip or palate reconstructions.

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