Inositol 1,4,5-trisphosphate receptor function in human oocytes: Calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP3 are blocked by a specific antibody to the type I receptor

P. T. Goud, A. P. Goud, L. Leybaert, P. Van Oostveldt, K. Mikoshiba, M. P. Diamond, M. Dhont

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Type I inositol 1,4,5-trisphosphate-sensitive receptors (InsP3R) are expressed in human oocytes and may be involved in operating the Ca2+ release triggered by the fertilizing sperm. This study examines the contribution of type I InSP3R in operating Ca2+ release in human oocytes secondary to InsP3 itself, using a specific function-blocking antibody in conjunction with photolytic release of microinjected InsP3. Intracellular Ca2+ responses were assessed in oocytes microinjected with only caged InsP3 in experiment set A, while in experiment sets B and C, sibling oocytes were injected with caged InsP3 and the blocking antibody or a corresponding volume of medium, prior to flash photolysis. In experiment set C, certain fertilization-related phenomena (cortical granule exocytosis and chromatin configurations) were assessed using optical sections and three-dimensional image reconstructions obtained from a confocal laser scanning microscope. In experiment set A, photolytic release of InsP3 triggered a Ca2+ response (increase from ∼100 to 220 nmol/l followed by an exponential recovery, n = 8) and a wave in the oocytes that spread from the stimulation point to the opposite pole. In set B, photolytic InsP3 release generated Ca2+ responses in control oocytes (n = 9), but not in the antibody-injected oocytes (n = 7). In set C, cortical granule exocytosis and anaphase chromosome configurations were noted in the control oocytes; after flash photolysis (n = 6). These changes were completely absent in antibody injected oocytes as their cortical granules were intact and the chromosomes were in metaphase. These oocytes had also lacked Ca2+ responses as in set B (n = 5). This study demonstrates the functional presence of type I InsP3R-operated Ca2+ channels in human oocytes and further suggests an active role of InsP3 in triggering the Ca2+ rise and secondary activation phenomena at fertilization.

Original languageEnglish (US)
Pages (from-to)912-918
Number of pages7
JournalMolecular Human Reproduction
Volume8
Issue number10
StatePublished - Oct 1 2002
Externally publishedYes

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Inositol 1,4,5-Trisphosphate Receptors
Oocytes
Calcium
Antibodies
Blocking Antibodies
Photolysis
Exocytosis
Fertilization
Chromosomes
Anaphase
Computer-Assisted Image Processing
Three-Dimensional Imaging
Metaphase
Chromatin
Spermatozoa
Lasers

Keywords

  • Ca transients
  • Caged IP
  • Fertilization
  • Flash photolysis
  • Inositol 1,4,5-trisphosphate receptors

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Cell Biology

Cite this

Inositol 1,4,5-trisphosphate receptor function in human oocytes : Calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP3 are blocked by a specific antibody to the type I receptor. / Goud, P. T.; Goud, A. P.; Leybaert, L.; Van Oostveldt, P.; Mikoshiba, K.; Diamond, M. P.; Dhont, M.

In: Molecular Human Reproduction, Vol. 8, No. 10, 01.10.2002, p. 912-918.

Research output: Contribution to journalArticle

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