Inositol(1,3,4,5) tetrakisphosphate plays an important role in calcium mobilization from Entamoeba histolytica

Sanghamitra Raha, Banabihari Giri, Bhudeb Bhattacharyya, Birendra B. Biswas

Research output: Contribution to journalArticle

15 Scopus citations


Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3,4,5)tetrakis-phosphate (Ins(1,3,4,5)P4). Similarly, prior depletion of Ins(1,3,4,5)P4-sensitive stores did not have any influence on subsequent calcium release by Ins(1,4,5)P3. The EC50 for calcium release was 0.15 μM with Ins(1,4,5)P3 and 0.68 μM with Ins(1,3,4,5)P4. In conclusion, the Ins(1,3,4,5)P4-sensitive calcium store in E. histolytica is separate and independent from the Ins(1,4,5)P3-sensitive pool.

Original languageEnglish (US)
Pages (from-to)316-318
Number of pages3
JournalFEBS Letters
Issue number3
Publication statusPublished - Apr 10 1995
Externally publishedYes



  • Calcium
  • Entamoeba histolytica
  • Fura-2
  • Inositol(1,3,4,5)tetrakis-phosphate
  • Inositol(1,4,5)trisphosphate

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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