Instability of miRNA and cDNAs derivatives in RNA preparations

Valia Bravo-Egana, Samuel Rosero, Camillo Ricordi, Ricardo L. Pastori

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.

Original languageEnglish (US)
Pages (from-to)1052-1055
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume353
Issue number4
DOIs
StatePublished - Feb 23 2007
Externally publishedYes

Fingerprint

MicroRNAs
Complementary DNA
RNA
Derivatives
Molecules
Regulator Genes
Gene expression
Gene Expression
Degradation

Keywords

  • MiRNA cDNA stability
  • MicroRNA isolation
  • MicroRNA stability
  • Quantification of microRNAs

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Instability of miRNA and cDNAs derivatives in RNA preparations. / Bravo-Egana, Valia; Rosero, Samuel; Ricordi, Camillo; Pastori, Ricardo L.

In: Biochemical and Biophysical Research Communications, Vol. 353, No. 4, 23.02.2007, p. 1052-1055.

Research output: Contribution to journalArticle

Bravo-Egana, Valia ; Rosero, Samuel ; Ricordi, Camillo ; Pastori, Ricardo L. / Instability of miRNA and cDNAs derivatives in RNA preparations. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 353, No. 4. pp. 1052-1055.
@article{8d2b573bcb644fdcabbb0f1f8fea6057,
title = "Instability of miRNA and cDNAs derivatives in RNA preparations",
abstract = "Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.",
keywords = "MiRNA cDNA stability, MicroRNA isolation, MicroRNA stability, Quantification of microRNAs",
author = "Valia Bravo-Egana and Samuel Rosero and Camillo Ricordi and Pastori, {Ricardo L.}",
year = "2007",
month = "2",
day = "23",
doi = "10.1016/j.bbrc.2006.12.135",
language = "English (US)",
volume = "353",
pages = "1052--1055",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Instability of miRNA and cDNAs derivatives in RNA preparations

AU - Bravo-Egana, Valia

AU - Rosero, Samuel

AU - Ricordi, Camillo

AU - Pastori, Ricardo L.

PY - 2007/2/23

Y1 - 2007/2/23

N2 - Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.

AB - Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.

KW - MiRNA cDNA stability

KW - MicroRNA isolation

KW - MicroRNA stability

KW - Quantification of microRNAs

UR - http://www.scopus.com/inward/record.url?scp=33846131225&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846131225&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2006.12.135

DO - 10.1016/j.bbrc.2006.12.135

M3 - Article

C2 - 17204243

AN - SCOPUS:33846131225

VL - 353

SP - 1052

EP - 1055

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -