Instability of miRNA and cDNAs derivatives in RNA preparations

Valia Bravo, Samuel Rosero, Camillo Ricordi, Ricardo L. Pastori

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Micro RNAs (miRNAs) are small RNA molecules, which function as important regulators of gene expression. We found that RNA preparation methods commonly utilized for miRNA expression studies yield highly unstable miRNAs. We studied the stability of four miRNAs belonging to different miRNAs families. A significant degradation of these molecules may be observed already three days after RNA isolation. Moreover, the respective cDNAs are highly unstable as well. Our findings indicate that instability of miRNAs and their cDNAs should be considered when designing miRNA expression studies.

Original languageEnglish (US)
Pages (from-to)1052-1055
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume353
Issue number4
DOIs
StatePublished - Feb 23 2007
Externally publishedYes

Keywords

  • MiRNA cDNA stability
  • MicroRNA isolation
  • MicroRNA stability
  • Quantification of microRNAs

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Instability of miRNA and cDNAs derivatives in RNA preparations'. Together they form a unique fingerprint.

  • Cite this