Insulin and IGF-1 induced vasodilation in canine coronary microvessels

C. L. Oltman, D. D. Gutterman, K. A. Quinn, R. S. Bar, Kevin C Dellsperger

Research output: Contribution to journalArticle

Abstract

Insulin and insulin-like growth factor 1 (IGF-1) are structurally related peptides capable of stimulating a variety of metabolic and mitogenic processes. Insulin has been demonstrated to cause dilatation in a variety of vascular tissues. The purpose of this study was to determine mechanisms involved in coronary microvascular dilation to insulin and IGF-1. Epicardial coronary arterioles (diameter = 101.8 ± 9.2 μm) from 24 mongrel dogs (5-8 kg) were mounted onto pipettes in warmed (37° C), oxygenated (20% O2, 75% N2 and 5% CO2) Kreb's buffer (distending pressure 20 mmHg, no flow). Vessels were incubated in control solution, L-NNA (10 μM), glibenclamide (1 μM), indomethacin (10 μM), or tetraethylammonium chloride (TEA, 1 mM) for 30 minutes. After constriction with endothelin (30-50% of resting diameter) cumulative doses of insulin and IGF-1 (0.1 - 100 ng/ml), were added extraluminally to the bath and internal diameters measured by video microscopy. Results: Insulin and IGF-1 produced similar relaxation in coronary arterioles (Max. relaxation 83.5 ± 19.1 and 63.64 ± 13.45 respectively). L-NNA, glibenclamide, indomethacin or TEA did not alter the response to insulin or IGF-1. We conclude that in canine coronary microvessels, relaxation to insulin and IGF-1 is similar and not mediated by nitric oxide, prostacyclin, or select K+ channels.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - Dec 1 1997

Fingerprint

vasodilation
somatomedins
Somatomedins
Microvessels
Vasodilation
Canidae
insulin
Insulin
dogs
glibenclamide
indomethacin
Glyburide
Arterioles
Indomethacin
Dilatation
prostacyclin
Video Microscopy
endothelins
Tetraethylammonium
Endothelins

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Oltman, C. L., Gutterman, D. D., Quinn, K. A., Bar, R. S., & Dellsperger, K. C. (1997). Insulin and IGF-1 induced vasodilation in canine coronary microvessels. FASEB Journal, 11(3).

Insulin and IGF-1 induced vasodilation in canine coronary microvessels. / Oltman, C. L.; Gutterman, D. D.; Quinn, K. A.; Bar, R. S.; Dellsperger, Kevin C.

In: FASEB Journal, Vol. 11, No. 3, 01.12.1997.

Research output: Contribution to journalArticle

Oltman, CL, Gutterman, DD, Quinn, KA, Bar, RS & Dellsperger, KC 1997, 'Insulin and IGF-1 induced vasodilation in canine coronary microvessels', FASEB Journal, vol. 11, no. 3.
Oltman CL, Gutterman DD, Quinn KA, Bar RS, Dellsperger KC. Insulin and IGF-1 induced vasodilation in canine coronary microvessels. FASEB Journal. 1997 Dec 1;11(3).
Oltman, C. L. ; Gutterman, D. D. ; Quinn, K. A. ; Bar, R. S. ; Dellsperger, Kevin C. / Insulin and IGF-1 induced vasodilation in canine coronary microvessels. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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