Interaction and fusion of unilamellar vesicles containing cerebrosides and sulfatides induced by myelin basic protein

Bruno Maggio, Robert K Yu

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The effects of myelin basic protein on the aggregation, lipid bilayer merging, intercommunication of aqueous compartments and leakage of small unilamellar vesicles of egg phosphatidylcholine containing different proportions of galactocerebroside and sulfatide were investigated. This was performed employing light scattering, absorbance changes and fluorescence assays (resonance energy transfer, Terbium/dipicolinic acid asssay and carboxyfluorescein release). The apposition of membranes rapidly induced by myelin basic protein is enhanced by sulfatide but reduced by galactocerebroside compared to vesicles of egg phosphatidylcholine alone. On the other hand, the presence of either glycosphingolipid in the membrane interferes with the induction by myelin basic protein of lipid bilayer merging, subsequent fusion and changes of the membrane permeability. Our results support an important modulation by sulfatide and galactocerebroside on the interactions among membranes induced by myelin basic protein, depending on the relative proportions of the glycosphingolipids and phosphatidylcholine.

Original languageEnglish (US)
Pages (from-to)127-136
Number of pages10
JournalChemistry and Physics of Lipids
Volume51
Issue number2
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Cerebrosides
Sulfoglycosphingolipids
Unilamellar Liposomes
Myelin Basic Protein
Fusion reactions
Phosphatidylcholines
Membranes
Glycosphingolipids
Lipid bilayers
Lipid Bilayers
Merging
Ovum
Terbium
Fluorescence Resonance Energy Transfer
Membrane Fusion
Light scattering
Energy transfer
Permeability
Assays
Agglomeration

Keywords

  • cerebrosides
  • membrane fusion
  • membrane interactions
  • myelin basic protein
  • myelin stability
  • sulfatide

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Cell Biology

Cite this

Interaction and fusion of unilamellar vesicles containing cerebrosides and sulfatides induced by myelin basic protein. / Maggio, Bruno; Yu, Robert K.

In: Chemistry and Physics of Lipids, Vol. 51, No. 2, 01.01.1989, p. 127-136.

Research output: Contribution to journalArticle

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N2 - The effects of myelin basic protein on the aggregation, lipid bilayer merging, intercommunication of aqueous compartments and leakage of small unilamellar vesicles of egg phosphatidylcholine containing different proportions of galactocerebroside and sulfatide were investigated. This was performed employing light scattering, absorbance changes and fluorescence assays (resonance energy transfer, Terbium/dipicolinic acid asssay and carboxyfluorescein release). The apposition of membranes rapidly induced by myelin basic protein is enhanced by sulfatide but reduced by galactocerebroside compared to vesicles of egg phosphatidylcholine alone. On the other hand, the presence of either glycosphingolipid in the membrane interferes with the induction by myelin basic protein of lipid bilayer merging, subsequent fusion and changes of the membrane permeability. Our results support an important modulation by sulfatide and galactocerebroside on the interactions among membranes induced by myelin basic protein, depending on the relative proportions of the glycosphingolipids and phosphatidylcholine.

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