Interaction of anionic cephalosporins with the intestinal and renal peptide transportrs PEPT 1 and PEPT 2

Malliga E. Ganapathy, Puttur D Prasad, Bryan Mackenzie, Vadivel Ganapathy, Frederick H. Leibach

Research output: Contribution to journalArticle

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Abstract

The present study was undertaken to investigate the interaction of anionic cephalosporins (cefixime, ceftibuten, and cefdinir) with the renal peptide transporter (PEPT 2) and the intestinal peptide transporter (PEPT 1) using four different experimental model systems. In the first approach, the human colon carcinoma cell line Caco-2 which expresses PEPT 1 and the SHR rat kidney cell line SKPT which expresses PEPT 2 were used. The uptake of the dipeptide Gly-Sar mediated by PEPT 1 or PEPT 2 in these cells was inhibited significantly by the anionic cephalosporins, with the following order of potency: ceftibuten > cefixime > cefdinir. The inhibition was competitive in nature. Even though the order of potency was the same for PEPT 1 and PEPT 2, PEPT 1 exhibited much lesser sensitivity to inhibition than PEPT 2. In the second approach, the cloned human PEPT 1 and PEPT 2 were functionally expressed in HeLa cells following which the cells were used to study the interaction of anionic cephalosporins with PEPT 1 and PEPT 2. Again, Gly-Sar uptake mediated by the human PEPT 1 and PEPT 2 in HeLa cells was found to be inhibited by the anionic cephalosporins with the same order potency as in Caco-2 and SKPT cells. In the third approach, brush border membrane vesicles isolated from rat kidneys were employed. In this approach also it was found that PEPT 2-mediated Gly-Sar uptake was inhibited by cefixime and ceftibuten. In the fourth approach, the human PEPT 1 was expressed in Xenopus laevis oocytes and PEPT 1-mediated transport of ceftibuten was investigated directly by electrophysiological methods. Ceftibuten evoked inward currents in PEPT 1-expressing oocytes but not in water-injected oocytes, showing that the transport of the anionic cephalosporin via PEPT 1 is associated with transfer of positive charge. The ceftibuten-evoked currents were saturable with respect to ceftibuten concentration and were markedly dependent on membrane potential. It is concluded that anionic cephalosporins interact with the peptide transporters expressed in the intestine (PEPT 1) as well as in the kidney (PEPT 2).

Original languageEnglish (US)
Pages (from-to)296-308
Number of pages13
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1324
Issue number2
DOIs
StatePublished - Mar 13 1997

Fingerprint

Cephalosporins
Kidney
Peptides
cefdinir
Cefixime
1,10-phenanthroline-platinum(II)-ethylenediamine
Oocytes
HeLa Cells
Rats
Cells
Membranes
Cell Line

Keywords

  • Caco-2 cell
  • Cephalosporin
  • Intestine
  • Kidney
  • Peptide transporter
  • SKPT cell

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Interaction of anionic cephalosporins with the intestinal and renal peptide transportrs PEPT 1 and PEPT 2. / Ganapathy, Malliga E.; Prasad, Puttur D; Mackenzie, Bryan; Ganapathy, Vadivel; Leibach, Frederick H.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1324, No. 2, 13.03.1997, p. 296-308.

Research output: Contribution to journalArticle

Ganapathy, Malliga E. ; Prasad, Puttur D ; Mackenzie, Bryan ; Ganapathy, Vadivel ; Leibach, Frederick H. / Interaction of anionic cephalosporins with the intestinal and renal peptide transportrs PEPT 1 and PEPT 2. In: Biochimica et Biophysica Acta - Biomembranes. 1997 ; Vol. 1324, No. 2. pp. 296-308.
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