Interactions between PBEF and oxidative stress proteins - A potential new mechanism underlying PBEF in the pathogenesis of acute lung injury

Li Qin Zhang, Djanybek M. Adyshev, Patrick Singleton, Hailong Li, Javier Cepeda, Sheng You Huang, Xiaoqin Zou, Alexander D. Verin, Jiancheng Tu, Joe G.N. Garcia, Shui Qing Ye

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19 Scopus citations

Abstract

Identification of pre-B-cell colony-enhancing factor (PBEF) interacting partners may reveal new molecular mechanisms of PBEF in the pathogenesis of acute lung injury (ALI). The interactions between PBEF and NADH dehydrogenase subunit 1(ND1), ferritin light chain and interferon induced transmembrane 3 (IFITM3) in human pulmonary vascular endothelial cells were identified and validated. ND1, ferritin and IFITM3 are involved in oxidative stress and inflammation. Overexpression of PBEF increased its interactions and intracellular oxidative stress, which can be attenuated by rotenone. The interaction modeling between PBEF and ND1 is consistent with the corresponding experimental finding. These interactions may underlie a novel role of PBEF in the pathogenesis of ALI. Structured summary: MINT-6538697: PBEF (uniprotkb:P43490) physically interacts (MI:0218) with NADH1 (uniprotkb:P03886) by two hybrid (MI:0018)MINT-6538811, MINT-6538868: PBEF (uniprotkb:P43490) physically interacts (MI:0218) with interferon-induced transmembrane protein 3 (uniprotkb:Q01628) by anti bait coimmunoprecipitation (MI:0006)MINT-6538787, MINT-6538841: PBEF (uniprotkb:P43490) physically interacts (MI:0218) with NADH1 (uniprotkb:P03886) by anti bait coimmunoprecipitation (MI:0006)MINT-6538755: PBEF (uniprotkb:P43490) physically interacts (MI:0218) with γ-glutamyl-transferase (uniprotkb:P19440) by two hybrid (MI:0018)MINT-6538799, MINT-6538862: PBEF (uniprotkb:P43490) physically interacts (MI:0218)with Ferritin light chain (uniprotkb:P02792) by anti bait coimmunoprecipitation (MI:0006)MINT-6538769: PBEF (uniprotkb:P43490) physically interacts (MI:0218) with E2L6 (uniprotkb:O14933) by two hybrid (MI:0018)MINT-6538741: PBEF (uniprotkb:P43490) physically interacts (MI:0218) with Adenosine A2aR (uniprotkb:P29274) by two hybrid (MI:0018)MINT-6538727: PBEF (uniprotkb:P43490) physically interacts (MI:0218) with interferon-induced transmembrane protein 3 (uniprotkb:Q01628) by two hybrid (MI:0018)MINT-6538712: PBEF (uniprotkb:P43490) physically interacts (MI:0218) with Ferritin light chain (uniprotkb:P02792) by two hybrid (MI:0018).

Original languageEnglish (US)
Pages (from-to)1802-1808
Number of pages7
JournalFEBS Letters
Volume582
Issue number13
DOIs
StatePublished - Jun 11 2008

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Keywords

  • Acute lung injury
  • Inflammation
  • Interaction
  • Oxidative stress
  • PBEF
  • Vascular permeability

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Zhang, L. Q., Adyshev, D. M., Singleton, P., Li, H., Cepeda, J., Huang, S. Y., Zou, X., Verin, A. D., Tu, J., Garcia, J. G. N., & Ye, S. Q. (2008). Interactions between PBEF and oxidative stress proteins - A potential new mechanism underlying PBEF in the pathogenesis of acute lung injury. FEBS Letters, 582(13), 1802-1808. https://doi.org/10.1016/j.febslet.2008.04.061