Interfering with the actin network and its effect on long-term potentiation and synaptic tagging in hippocampal CA1 neurons in slices in vitro

Binu Ramachandran, Julietta Uta Frey

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Long-term potentiation (LTP) is a cellular correlate formemoryformation, which requires the dynamic changes of the actin cytoskeleton. As shown by others, the polymerization of the actin network is important for early stages of LTP. Here, we investigated the role of actin dynamics in synaptic tagging and particularly in the induction of protein synthesis-dependent late-LTP in the CA1 region in hippocampal slices in vitro.We found that the inhibition of actin polymerization affects protein synthesis-independent early-LTP, prevents late-LTP, and interferes with synaptic tagging in apical dendrites of hippocampal CA1. The transformation of early-LTP into late-LTP was blocked by the application of the structurally different actin polymerization inhibitors latrunculin A or cytochalasin D. We suggest that the actin network is required for early "housekeeping" processes to induce and maintain early-LTP. Furthermore, inhibition of actin dynamics negatively interacts with the setting of the synaptic tagging complex. We propose actin as a further tag-specific molecule in apical CA1 dendrites where it is directly involved in the tagging/capturing machinery. Consequently, inhibition of the actin network prevents the interaction of tagging complexes with plasticity-related proteins. This results in the prevention of late-LTP by inhibition of the actin network during LTP induction.

Original languageEnglish (US)
Pages (from-to)12167-12173
Number of pages7
JournalJournal of Neuroscience
Volume29
Issue number39
DOIs
StatePublished - Sep 30 2009

Fingerprint

Long-Term Potentiation
Actins
Neurons
Polymerization
Dendrites
In Vitro Techniques
Hippocampal CA1 Region
Cytochalasin D
Housekeeping
Proteins
Actin Cytoskeleton

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Interfering with the actin network and its effect on long-term potentiation and synaptic tagging in hippocampal CA1 neurons in slices in vitro. / Ramachandran, Binu; Frey, Julietta Uta.

In: Journal of Neuroscience, Vol. 29, No. 39, 30.09.2009, p. 12167-12173.

Research output: Contribution to journalArticle

@article{5d89523450d7443e8c55bcb1cc3a0000,
title = "Interfering with the actin network and its effect on long-term potentiation and synaptic tagging in hippocampal CA1 neurons in slices in vitro",
abstract = "Long-term potentiation (LTP) is a cellular correlate formemoryformation, which requires the dynamic changes of the actin cytoskeleton. As shown by others, the polymerization of the actin network is important for early stages of LTP. Here, we investigated the role of actin dynamics in synaptic tagging and particularly in the induction of protein synthesis-dependent late-LTP in the CA1 region in hippocampal slices in vitro.We found that the inhibition of actin polymerization affects protein synthesis-independent early-LTP, prevents late-LTP, and interferes with synaptic tagging in apical dendrites of hippocampal CA1. The transformation of early-LTP into late-LTP was blocked by the application of the structurally different actin polymerization inhibitors latrunculin A or cytochalasin D. We suggest that the actin network is required for early {"}housekeeping{"} processes to induce and maintain early-LTP. Furthermore, inhibition of actin dynamics negatively interacts with the setting of the synaptic tagging complex. We propose actin as a further tag-specific molecule in apical CA1 dendrites where it is directly involved in the tagging/capturing machinery. Consequently, inhibition of the actin network prevents the interaction of tagging complexes with plasticity-related proteins. This results in the prevention of late-LTP by inhibition of the actin network during LTP induction.",
author = "Binu Ramachandran and Frey, {Julietta Uta}",
year = "2009",
month = "9",
day = "30",
doi = "10.1523/JNEUROSCI.2045-09.2009",
language = "English (US)",
volume = "29",
pages = "12167--12173",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "39",

}

TY - JOUR

T1 - Interfering with the actin network and its effect on long-term potentiation and synaptic tagging in hippocampal CA1 neurons in slices in vitro

AU - Ramachandran, Binu

AU - Frey, Julietta Uta

PY - 2009/9/30

Y1 - 2009/9/30

N2 - Long-term potentiation (LTP) is a cellular correlate formemoryformation, which requires the dynamic changes of the actin cytoskeleton. As shown by others, the polymerization of the actin network is important for early stages of LTP. Here, we investigated the role of actin dynamics in synaptic tagging and particularly in the induction of protein synthesis-dependent late-LTP in the CA1 region in hippocampal slices in vitro.We found that the inhibition of actin polymerization affects protein synthesis-independent early-LTP, prevents late-LTP, and interferes with synaptic tagging in apical dendrites of hippocampal CA1. The transformation of early-LTP into late-LTP was blocked by the application of the structurally different actin polymerization inhibitors latrunculin A or cytochalasin D. We suggest that the actin network is required for early "housekeeping" processes to induce and maintain early-LTP. Furthermore, inhibition of actin dynamics negatively interacts with the setting of the synaptic tagging complex. We propose actin as a further tag-specific molecule in apical CA1 dendrites where it is directly involved in the tagging/capturing machinery. Consequently, inhibition of the actin network prevents the interaction of tagging complexes with plasticity-related proteins. This results in the prevention of late-LTP by inhibition of the actin network during LTP induction.

AB - Long-term potentiation (LTP) is a cellular correlate formemoryformation, which requires the dynamic changes of the actin cytoskeleton. As shown by others, the polymerization of the actin network is important for early stages of LTP. Here, we investigated the role of actin dynamics in synaptic tagging and particularly in the induction of protein synthesis-dependent late-LTP in the CA1 region in hippocampal slices in vitro.We found that the inhibition of actin polymerization affects protein synthesis-independent early-LTP, prevents late-LTP, and interferes with synaptic tagging in apical dendrites of hippocampal CA1. The transformation of early-LTP into late-LTP was blocked by the application of the structurally different actin polymerization inhibitors latrunculin A or cytochalasin D. We suggest that the actin network is required for early "housekeeping" processes to induce and maintain early-LTP. Furthermore, inhibition of actin dynamics negatively interacts with the setting of the synaptic tagging complex. We propose actin as a further tag-specific molecule in apical CA1 dendrites where it is directly involved in the tagging/capturing machinery. Consequently, inhibition of the actin network prevents the interaction of tagging complexes with plasticity-related proteins. This results in the prevention of late-LTP by inhibition of the actin network during LTP induction.

UR - http://www.scopus.com/inward/record.url?scp=70349631642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349631642&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.2045-09.2009

DO - 10.1523/JNEUROSCI.2045-09.2009

M3 - Article

C2 - 19793974

AN - SCOPUS:70349631642

VL - 29

SP - 12167

EP - 12173

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 39

ER -