Interferon-beta-1beta protects against multiple sclerosis-induced endothelial cells apoptosis

Shaghayegh Haghjooy Javanmard, M. Mohammad Saadatnia, V. Vida Homayouni, M. Mahin Nikoogoftar, Amir H. Maghzi, M. Etemadifar, V. G. Chaitanya, Jeanie C. McGee, Alireza Minagar, J. Steven Alexander

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Disruption of the blood-brain-barrier (BBB) due to endothelial cell (EC) injury is an essential step in formation of multiple sclerosis (MS) lesions. We investigated the role of endothelial cell (EC) apoptosis in the pathophysiology of MS, studying the therapeutic effect of IFN-beta-1b against MS sera-induced endothelial apoptosis. Human umbilical vein endothelial cells were treated with sera from patients with active MS (in relapse), MS in remission, or sera from healthy volunteers (each n = 5). Apoptosis was assessed by annexin V-propidium iodide staining. Effects of IFN-beta-1b on EC apoptosis were tested at increasing doses (10, 100, and1000 U/ml). Nitrite (NO2--) levels were determined in culture supernatants. EC apoptosis was increased by sera from exacerbating MS patients, but not remission, compared to healthy individuals (p<0.001). Effects were blocked by IFN-beta-1b at 10U/ml (p<0.05), but not higher doses, and was associated with increased NO/NO2- production (p<0.05). EC apoptosis leading to disruption of the BBB may play a role in MS etiology and represents a novel therapeutic mechanism of action for IFN-beta-1b in MS therapy.

Original languageEnglish (US)
Pages (from-to)1368-1374
Number of pages7
JournalFrontiers in Bioscience - Elite
Volume4 E
Issue number4
StatePublished - Jan 1 2012
Externally publishedYes

Keywords

  • Apoptosis
  • Endothelial Cell
  • Interferon-Beta
  • Multiple sclerosis
  • Nitric Oxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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