Interferon-beta-1beta protects against multiple sclerosis-induced endothelial cells apoptosis

Shaghayegh Haghjooy Javanmard; M. Mohammad Saadatnia; V. Vida Homayouni; M. Mahin Nikoogoftar; Amir H. Maghzi; M. Etemadifar; V. G. Chaitanya; Jeanie C. McGee; Alireza Minagar; J. Steven Alexander

Interferon-beta-1beta protects against multiple sclerosis-induced endothelial cells apoptosis

Shaghayegh Haghjooy Javanmard, M. Mohammad Saadatnia, V. Vida Homayouni, M. Mahin Nikoogoftar, Amir H. Maghzi, M. Etemadifar, V. G. Chaitanya, Jeanie C. McGee, Alireza Minagar, J. Steven Alexander

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Disruption of the blood-brain-barrier (BBB) due to endothelial cell (EC) injury is an essential step in formation of multiple sclerosis (MS) lesions. We investigated the role of endothelial cell (EC) apoptosis in the pathophysiology of MS, studying the therapeutic effect of IFN-beta-1b against MS sera-induced endothelial apoptosis. Human umbilical vein endothelial cells were treated with sera from patients with active MS (in relapse), MS in remission, or sera from healthy volunteers (each n = 5). Apoptosis was assessed by annexin V-propidium iodide staining. Effects of IFN-beta-1b on EC apoptosis were tested at increasing doses (10, 100, and1000 U/ml). Nitrite (NO₂-^-) levels were determined in culture supernatants. EC apoptosis was increased by sera from exacerbating MS patients, but not remission, compared to healthy individuals (p<0.001). Effects were blocked by IFN-beta-1b at 10U/ml (p<0.05), but not higher doses, and was associated with increased NO/NO2- production (p<0.05). EC apoptosis leading to disruption of the BBB may play a role in MS etiology and represents a novel therapeutic mechanism of action for IFN-beta-1b in MS therapy.

Original language	English (US)
Pages (from-to)	1368-1374
Number of pages	7
Journal	Frontiers in Bioscience - Elite
Volume	4 E
Issue number	4
State	Published - Jan 1 2012
Externally published	Yes

Keywords

Apoptosis
Endothelial Cell
Interferon-Beta
Multiple sclerosis
Nitric Oxide

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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title = "Interferon-beta-1beta protects against multiple sclerosis-induced endothelial cells apoptosis",

abstract = "Disruption of the blood-brain-barrier (BBB) due to endothelial cell (EC) injury is an essential step in formation of multiple sclerosis (MS) lesions. We investigated the role of endothelial cell (EC) apoptosis in the pathophysiology of MS, studying the therapeutic effect of IFN-beta-1b against MS sera-induced endothelial apoptosis. Human umbilical vein endothelial cells were treated with sera from patients with active MS (in relapse), MS in remission, or sera from healthy volunteers (each n = 5). Apoptosis was assessed by annexin V-propidium iodide staining. Effects of IFN-beta-1b on EC apoptosis were tested at increasing doses (10, 100, and1000 U/ml). Nitrite (NO2--) levels were determined in culture supernatants. EC apoptosis was increased by sera from exacerbating MS patients, but not remission, compared to healthy individuals (p<0.001). Effects were blocked by IFN-beta-1b at 10U/ml (p<0.05), but not higher doses, and was associated with increased NO/NO2- production (p<0.05). EC apoptosis leading to disruption of the BBB may play a role in MS etiology and represents a novel therapeutic mechanism of action for IFN-beta-1b in MS therapy.",

keywords = "Apoptosis, Endothelial Cell, Interferon-Beta, Multiple sclerosis, Nitric Oxide",

author = "Javanmard, {Shaghayegh Haghjooy} and {Mohammad Saadatnia}, M. and {Vida Homayouni}, V. and {Mahin Nikoogoftar}, M. and Maghzi, {Amir H.} and M. Etemadifar and Chaitanya, {V. G.} and McGee, {Jeanie C.} and Alireza Minagar and Alexander, {J. Steven}",

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AU - Javanmard, Shaghayegh Haghjooy

AU - Mohammad Saadatnia, M.

AU - Vida Homayouni, V.

AU - Mahin Nikoogoftar, M.

AU - Maghzi, Amir H.

AU - Etemadifar, M.

AU - Chaitanya, V. G.

AU - McGee, Jeanie C.

AU - Minagar, Alireza

AU - Alexander, J. Steven

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AB - Disruption of the blood-brain-barrier (BBB) due to endothelial cell (EC) injury is an essential step in formation of multiple sclerosis (MS) lesions. We investigated the role of endothelial cell (EC) apoptosis in the pathophysiology of MS, studying the therapeutic effect of IFN-beta-1b against MS sera-induced endothelial apoptosis. Human umbilical vein endothelial cells were treated with sera from patients with active MS (in relapse), MS in remission, or sera from healthy volunteers (each n = 5). Apoptosis was assessed by annexin V-propidium iodide staining. Effects of IFN-beta-1b on EC apoptosis were tested at increasing doses (10, 100, and1000 U/ml). Nitrite (NO2--) levels were determined in culture supernatants. EC apoptosis was increased by sera from exacerbating MS patients, but not remission, compared to healthy individuals (p<0.001). Effects were blocked by IFN-beta-1b at 10U/ml (p<0.05), but not higher doses, and was associated with increased NO/NO2- production (p<0.05). EC apoptosis leading to disruption of the BBB may play a role in MS etiology and represents a novel therapeutic mechanism of action for IFN-beta-1b in MS therapy.

KW - Apoptosis

KW - Endothelial Cell

KW - Interferon-Beta

KW - Multiple sclerosis

KW - Nitric Oxide

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Interferon-beta-1beta protects against multiple sclerosis-induced endothelial cells apoptosis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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