Interleukin-1β Regulation of Adhesion Molecules on Human Gingival and Periodontal Ligament Fibroblasts

Brandon H. Joe, James L. Borke, Meral Keskintepe, Philip Jerry Hanes, Jason M. Mailhot, Baldev B. Singh

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19 Scopus citations

Abstract

Background: Adhesion molecules have been implicated in the pathogenesis of rheumatoid arthritis and may also play a role in the pathogenesis of periodontal disease by promoting the recruitment and retention of leukocytes in gingival tissue. Methods: The aim of the present study was to evaluate the capacity of interleukin-1β (IL-1β) to regulate adhesion molecule expression on clinically healthy human gingival (HGF) and periodontal ligament (PDL) fibroblasts. The HGF (n = 6) and PDL (n = 3) fibroblasts were treated with 1.0 ng/ml of IL-1β for 24 hours and then incubated with primary intercellular adhesion molecule-1 (ICAM-1) and vascular cellular adhesion molecule-1 (VCAM-1) antibodies followed by FITC-conjugated secondary antibodies. The expression of ICAM-1 and VCAM-1 was measured by immunofluorescence flow cytometry. Results: The levels of ICAM-1 expression in IL-1β treated HGF and PDL fibroblasts were statistically significant (P ≤0.05) compared to normal untreated controls using log-transformed data and 3-way analysis of variance. Both cells expressed VCAM-1 after IL-1β treatment, but the levels were not statistically different from controls. Conclusions: This study demonstrated that IL-1β upregulated ICAM-1 expression in both HGF and PDL fibroblasts. Even though the level of VCAM-1 was not statistically different from both HGF and PDL fibroblasts treated with IL-1β compared to controls, both cells do express the VCAM-1 molecules. These results suggest that ICAM-1 and VCAM-1 might be involved in the pathogenesis of periodontal disease.

Original languageEnglish (US)
Pages (from-to)865-870
Number of pages6
JournalJournal of Periodontology
Volume72
Issue number7
DOIs
StatePublished - Jul 1 2001

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Keywords

  • Fibroblasts
  • Intercellular adhesion molecule
  • Interleukin-1
  • Periodontal diseases/pathogenesis
  • Vascular cell adhesion molecule

ASJC Scopus subject areas

  • Periodontics

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