Interleukin-1 receptor antagonist gene polymorphism and obesity: A pilot study from north India

P K Manchanda, H K Bid, B R Achyut, B Mittal, N Srivastava, R D Mittal

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Abstract

The main adverse consequences of excess bodyweight are cardiovascular disease, type II diabetes, and several cancers, IL-1Ra serum concentration has been reported earlier to increase in human obesity and it is therefore assumed that the polymorphism of IL-1Ra may influence cytokine production. We designed this study to investigate whether the IL-1Ra polymorphism was associated with obesity. A total number of 103 individuals; 19 lean (BMI<25 Kg/m(2)), 51 overweight (BMI 25-29.9 Kg/m(2)) and 33 obese (BMI≥30.0 Kg/m(2)) were enrolled in this study. Genotyping was performed using a polymerase chain reaction PCR amplification of the intron-2 fragment harboring a variable number of tandem repeat (VNTR) nucleotide sequences 86 pb of tandem repeat. The PCR products were separated on 2% agarose gel. Statistical analysis was performed using SPSS software (version 11.5). We found no significant difference in genotype and allele frequencies between the three groups; lean vs. overweight and lean vs. obese (p=0.323; 0.202; 0.123 and 0.068 resp). However, an increased risk for obesity had a propensity to be higher in those having genotype II/II. This genotype has been reported to be a 'high producer' of IL-1Ra. Although no statistically significant relationship between IL-1Ra polymorphism and BMI was observed, however, a trend towards an increase of allele(*)II in overweight and obese group was observed. This may suggest that IL-1Ra appears to be induced by inflammatory stimuli as well as obesity-associated factors. This is relatively a pilot study: but nevertheless, may assist in identifying the pathophysiological cause for obesity.

Original languageEnglish (US)
Pages (from-to)61-4
Number of pages4
JournalIndian Journal of Clinical Biochemistry
Volume22
Issue number1
DOIs
StatePublished - Mar 2007

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Interleukin 1 Receptor Antagonist Protein
Interleukin-1 Receptors
Polymorphism
India
Obesity
Genes
Tandem Repeat Sequences
Genotype
Polymerase Chain Reaction
Minisatellite Repeats
Polymerase chain reaction
Medical problems
Gene Frequency
Sepharose
Introns
Type 2 Diabetes Mellitus
Amplification
Statistical methods
Cardiovascular Diseases
Software

Keywords

  • Journal Article

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Manchanda, P. K., Bid, H. K., Achyut, B. R., Mittal, B., Srivastava, N., & Mittal, R. D. (2007). Interleukin-1 receptor antagonist gene polymorphism and obesity: A pilot study from north India. Indian Journal of Clinical Biochemistry, 22(1), 61-4. https://doi.org/10.1007/BF02912883

Interleukin-1 receptor antagonist gene polymorphism and obesity : A pilot study from north India. / Manchanda, P K; Bid, H K; Achyut, B R; Mittal, B; Srivastava, N; Mittal, R D.

In: Indian Journal of Clinical Biochemistry, Vol. 22, No. 1, 03.2007, p. 61-4.

Research output: Contribution to journalArticle

Manchanda, PK, Bid, HK, Achyut, BR, Mittal, B, Srivastava, N & Mittal, RD 2007, 'Interleukin-1 receptor antagonist gene polymorphism and obesity: A pilot study from north India', Indian Journal of Clinical Biochemistry, vol. 22, no. 1, pp. 61-4. https://doi.org/10.1007/BF02912883
Manchanda, P K ; Bid, H K ; Achyut, B R ; Mittal, B ; Srivastava, N ; Mittal, R D. / Interleukin-1 receptor antagonist gene polymorphism and obesity : A pilot study from north India. In: Indian Journal of Clinical Biochemistry. 2007 ; Vol. 22, No. 1. pp. 61-4.
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