Interleukin-3 induces the phosphorylation of a distinct fraction of Bcl- 2

Paul Boyd Poommipanit, Bin Chen, Zoltán N. Oltvai

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Bcl-2-related proteins (i.e. Bcl-2 and Bax) regulate the effector stage of apoptosis and can modulate the entry of quiescent cells into the cell cycle. Phosphorylation of Bcl-2 is presumed to modify its apoptosis- inhibitory function. By utilizing an interleukin-3 (IL-3)-dependent hematopoietic cell line, we examined the structural requirements of Bcl-2 phosphorylation and the correlation of this post-translational modification with its function. In the presence of IL-3, constitutively expressed Bcl-2 was phosphorylated on serine residue(s), and phosphorylated Bcl-2 lost its capacity to heterodimerize with Bax. Whereas the majority of Bcl-2 resided in mitochondria, phosphorylation only affected a minor pool of total Bcl-2 that selectively partitioned into a soluble fraction. Cytosolic targeting of Bcl- 2 greatly increased its ratio of phosphorylation. Bcl-2 phosphorylation was reduced during IL-3 deprivation, and its phosphorylation was also delayed after transient cytokine deprivation. This pattern of phosphorylation temporally correlated with the accelerated exit and delayed reentry of Bcl- 2-expressing cells into the cell cycle upon transient IL-3 deprivation and subsequent cytokine restimulation. Thus, IL-3-induced phosphorylation of a distinct pool of Bcl-2 may contribute to the inactivation of its antiproliferative function.

Original languageEnglish (US)
Pages (from-to)1033-1039
Number of pages7
JournalJournal of Biological Chemistry
Volume274
Issue number2
DOIs
StatePublished - Jan 8 1999

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Phosphorylation
Interleukin-3
Cells
Cell Cycle
Apoptosis
Cytokines
Mitochondria
Reentry
Post Translational Protein Processing
Serine
Cell Line

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Interleukin-3 induces the phosphorylation of a distinct fraction of Bcl- 2. / Poommipanit, Paul Boyd; Chen, Bin; Oltvai, Zoltán N.

In: Journal of Biological Chemistry, Vol. 274, No. 2, 08.01.1999, p. 1033-1039.

Research output: Contribution to journalArticle

Poommipanit, Paul Boyd ; Chen, Bin ; Oltvai, Zoltán N. / Interleukin-3 induces the phosphorylation of a distinct fraction of Bcl- 2. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 2. pp. 1033-1039.
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