Interleukin-4 mediates STAT6 activation in 3T3-L1 preadipocytes but not adipocytes

Jianbei Deng, Kunjie Hua, Steven S. Lesser, Abigail H. Greiner, A. Whitney Walter, Mario B. Marrero, Joyce B. Harp

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

STAT6 is abundantly expressed in 3T3-L1 preadipocytes and adipocytes but activating ligands are not well defined. In this report, we provide evidence that interleukin 4 (IL-4) induced JAK2-mediated STAT6 tyrosine phosphorylation and DNA binding in 3T3-L1 preadipocytes but not in 3T3-L1 adipocytes. Loss of IL-4-mediated STAT6 tyrosine phosphorylation occurred 2 days after preadipocytes were induced to differentiate into adipocytes but when cells remained phenotypically preadipocytes. 3T3-L1 adipocytes were still responsive to IL-4 through tyrosine phosphorylation of other cellular proteins. We conclude that IL-4 signals through STAT6 in 3T3-L1 preadipocytes but not in 3T3-L1 adipocytes. This differentiation-dependent loss of STAT6 activation may be critical for distinct biological effects of IL-4 in 3T3-L1 preadipocytes and adipocytes. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)516-520
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume267
Issue number2
DOIs
StatePublished - Jan 19 2000
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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