Interleukin-4 mediates STAT6 activation in 3T3-L1 preadipocytes but not adipocytes

Jianbei Deng, Kunjie Hua, Steven S. Lesser, Abigail H. Greiner, A. Whitney Walter, Mario B Marrero, Joyce B. Harp

Research output: Contribution to journalArticle

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Abstract

STAT6 is abundantly expressed in 3T3-L1 preadipocytes and adipocytes but activating ligands are not well defined. In this report, we provide evidence that interleukin 4 (IL-4) induced JAK2-mediated STAT6 tyrosine phosphorylation and DNA binding in 3T3-L1 preadipocytes but not in 3T3-L1 adipocytes. Loss of IL-4-mediated STAT6 tyrosine phosphorylation occurred 2 days after preadipocytes were induced to differentiate into adipocytes but when cells remained phenotypically preadipocytes. 3T3-L1 adipocytes were still responsive to IL-4 through tyrosine phosphorylation of other cellular proteins. We conclude that IL-4 signals through STAT6 in 3T3-L1 preadipocytes but not in 3T3-L1 adipocytes. This differentiation-dependent loss of STAT6 activation may be critical for distinct biological effects of IL-4 in 3T3-L1 preadipocytes and adipocytes. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)516-520
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume267
Issue number2
DOIs
StatePublished - Jan 19 2000

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Adipocytes
Interleukin-4
Chemical activation
Phosphorylation
Tyrosine
Ligands
DNA
Proteins

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Deng, J., Hua, K., Lesser, S. S., Greiner, A. H., Walter, A. W., Marrero, M. B., & Harp, J. B. (2000). Interleukin-4 mediates STAT6 activation in 3T3-L1 preadipocytes but not adipocytes. Biochemical and Biophysical Research Communications, 267(2), 516-520. https://doi.org/10.1006/bbrc.1999.1993

Interleukin-4 mediates STAT6 activation in 3T3-L1 preadipocytes but not adipocytes. / Deng, Jianbei; Hua, Kunjie; Lesser, Steven S.; Greiner, Abigail H.; Walter, A. Whitney; Marrero, Mario B; Harp, Joyce B.

In: Biochemical and Biophysical Research Communications, Vol. 267, No. 2, 19.01.2000, p. 516-520.

Research output: Contribution to journalArticle

Deng, J, Hua, K, Lesser, SS, Greiner, AH, Walter, AW, Marrero, MB & Harp, JB 2000, 'Interleukin-4 mediates STAT6 activation in 3T3-L1 preadipocytes but not adipocytes', Biochemical and Biophysical Research Communications, vol. 267, no. 2, pp. 516-520. https://doi.org/10.1006/bbrc.1999.1993
Deng, Jianbei ; Hua, Kunjie ; Lesser, Steven S. ; Greiner, Abigail H. ; Walter, A. Whitney ; Marrero, Mario B ; Harp, Joyce B. / Interleukin-4 mediates STAT6 activation in 3T3-L1 preadipocytes but not adipocytes. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 267, No. 2. pp. 516-520.
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