Intermittent activation of peripheral chemoreceptors in awake rats induces Fos expression in rostral ventrolateral medulla-projecting neurons in the paraventricular nucleus of the hypothalamus

J. C. Cruz, L. G.H. Bonagamba, B. H. Machado, Vinicia Campana Biancardi, Javier Eduardo Stern

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Despite the well-established sympathoexcitation evoked by chemoreflex activation, the specific sub-regions of the CNS underlying such sympathetic responses remain to be fully characterized. In the present study we examined the effects of intermittent chemoreflex activation in awake rats on Fos-immunoreactivity (Fos-ir) in various subnuclei of the paraventricular nucleus of the hypothalamus (PVN), as well as in identified neurosecretory preautonomic PVN neurons. In response to intermittent chemoreflex activation, a significant increase in the number of Fos-ir cells was found in autonomic-related PVN subnuclei, including the posterior parvocellular, ventromedial parvocellular and dorsal-cap, but not in the neurosecretory magnocellular-containing lateral magnocellular subnucleus. No changes in Fos-ir following chemoreflex activation were observed in the anterior PVN subnucleus. Experiments combining Fos immunohistochemistry and neuronal tract tracing techniques showed a significant increase in Fos-ir in rostral ventrolateral medulla (RVLM)-projecting (PVN-RVLM), but not in nucleus of solitarii tract (NTS)-projecting PVN neurons. In summary, our results support the involvement of the PVN in the central neuronal circuitry activated in response to chemoreflex activation, and indicate that PVN-RVLM neurons constitute a neuronal substrate contributing to the sympathoexcitatory component of the chemoreflex.

Original languageEnglish (US)
Pages (from-to)463-472
Number of pages10
JournalNeuroscience
Volume157
Issue number2
DOIs
StatePublished - Nov 19 2008

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Paraventricular Hypothalamic Nucleus
Hypothalamus
Neurons
Anterior Hypothalamic Nucleus
Solitary Nucleus
Immunohistochemistry

Keywords

  • Fos protein
  • arterial chemoreceptors
  • chemoreflex
  • paraventricular nucleus
  • sympathetic activity

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Intermittent activation of peripheral chemoreceptors in awake rats induces Fos expression in rostral ventrolateral medulla-projecting neurons in the paraventricular nucleus of the hypothalamus. / Cruz, J. C.; Bonagamba, L. G.H.; Machado, B. H.; Biancardi, Vinicia Campana; Stern, Javier Eduardo.

In: Neuroscience, Vol. 157, No. 2, 19.11.2008, p. 463-472.

Research output: Contribution to journalArticle

Cruz, J. C. ; Bonagamba, L. G.H. ; Machado, B. H. ; Biancardi, Vinicia Campana ; Stern, Javier Eduardo. / Intermittent activation of peripheral chemoreceptors in awake rats induces Fos expression in rostral ventrolateral medulla-projecting neurons in the paraventricular nucleus of the hypothalamus. In: Neuroscience. 2008 ; Vol. 157, No. 2. pp. 463-472.
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