Intermittent fasting attenuates increases in neurogenesis after ischemia and reperfusion and improves recovery

Silvia Manzanero, Joanna R. Erion, Tomislav Santro, Frederik J. Steyn, Chen Chen, Thiruma V. Arumugam, Alexis M. Stranahan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Intermittent fasting (IF) is neuroprotective across a range of insults, but the question of whether extending the interval between meals alters neurogenesis after ischemia remains unexplored. We therefore measured cell proliferation, cell death, and neurogenesis after transient middle cerebral artery occlusion (MCAO) or sham surgery (SHAM) in mice fed ad libitum (AL) or maintained on IF for 3 months. IF was associated with twofold reductions in circulating levels of the adipocyte cytokine leptin in intact mice, but also prevented further reductions in leptin after MCAO. IF/MCAO mice also exhibit infarct volumes that were less than half those of AL/MCAO mice. We observed a 30% increase in basal cell proliferation in the hippocampus and subventricular zone (SVZ) in IF/SHAM, relative to AL/SHAM mice. However, cell proliferation after MCAO was limited in IF mice, which showed twofold increases in cell proliferation relative to IF/SHAM, whereas AL/MCAO mice exhibit fivefold increases relative to AL/SHAM. Attenuation of stroke-induced neurogenesis was correlated with reductions in cell death, with AL/MCAO mice exhibiting twice the number of dying cells relative to IF/MCAO mice. These observations indicate that IF protects against neurological damage in ischemic stroke, with circulating leptin as one possible mediator.

Original languageEnglish (US)
Pages (from-to)897-905
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume34
Issue number5
DOIs
StatePublished - Jan 1 2014

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Neurogenesis
Middle Cerebral Artery Infarction
Reperfusion
Fasting
Ischemia
Leptin
Cell Proliferation
Cell Death
Stroke
Lateral Ventricles
Adipocytes
Meals
Hippocampus
Cell Count
salicylhydroxamic acid
Cytokines

Keywords

  • caloric restriction
  • hippocampus
  • intermittent fasting
  • neurogenesis
  • stroke
  • subventricular zone

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Neurology

Cite this

Intermittent fasting attenuates increases in neurogenesis after ischemia and reperfusion and improves recovery. / Manzanero, Silvia; Erion, Joanna R.; Santro, Tomislav; Steyn, Frederik J.; Chen, Chen; Arumugam, Thiruma V.; Stranahan, Alexis M.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 34, No. 5, 01.01.2014, p. 897-905.

Research output: Contribution to journalArticle

Manzanero, Silvia ; Erion, Joanna R. ; Santro, Tomislav ; Steyn, Frederik J. ; Chen, Chen ; Arumugam, Thiruma V. ; Stranahan, Alexis M. / Intermittent fasting attenuates increases in neurogenesis after ischemia and reperfusion and improves recovery. In: Journal of Cerebral Blood Flow and Metabolism. 2014 ; Vol. 34, No. 5. pp. 897-905.
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