Intermittent fasting attenuates inflammasome activity in ischemic stroke

David Yang Wei Fann, Tomislav Santro, Silvia Manzanero, Alexander Widiapradja, Yi Lin Cheng, Seung Yoon Lee, Prasad Chunduri, Dong Gyu Jo, Alexis Michelle Stranahan, Mark P. Mattson, Thiruma V. Arumugam

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Recent findings have revealed a novel inflammatory mechanism that contributes to tissue injury in cerebral ischemia mediated by multi-protein complexes termed inflammasomes. Intermittent fasting (IF) can decrease the levels of pro-inflammatory cytokines in the periphery and brain. Here we investigated the impact of IF (16. h of food deprivation daily) for 4. months on NLRP1 and NLRP3 inflammasome activities following cerebral ischemia. Ischemic stroke was induced in C57BL/6J mice by middle cerebral artery occlusion, followed by reperfusion (I/R). IF decreased the activation of NF-κB and MAPK signaling pathways, the expression of NLRP1 and NLRP3 inflammasome proteins, and both IL-1β and IL-18 in the ischemic brain tissue. These findings demonstrate that IF can attenuate the inflammatory response and tissue damage following ischemic stroke by a mechanism involving suppression of NLRP1 and NLRP3 inflammasome activity.

Original languageEnglish (US)
Pages (from-to)114-119
Number of pages6
JournalExperimental Neurology
Volume257
DOIs
StatePublished - Jan 1 2014

Fingerprint

Inflammasomes
Fasting
Stroke
Brain Ischemia
Food Deprivation
Interleukin-18
Middle Cerebral Artery Infarction
Brain
Interleukin-1
Inbred C57BL Mouse
Reperfusion
Cytokines
Wounds and Injuries
Proteins

Keywords

  • Inflammasomes
  • Intermittent fasting
  • Ischemic stroke
  • NLRP1
  • NLRP3

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

Cite this

Fann, D. Y. W., Santro, T., Manzanero, S., Widiapradja, A., Cheng, Y. L., Lee, S. Y., ... Arumugam, T. V. (2014). Intermittent fasting attenuates inflammasome activity in ischemic stroke. Experimental Neurology, 257, 114-119. https://doi.org/10.1016/j.expneurol.2014.04.017

Intermittent fasting attenuates inflammasome activity in ischemic stroke. / Fann, David Yang Wei; Santro, Tomislav; Manzanero, Silvia; Widiapradja, Alexander; Cheng, Yi Lin; Lee, Seung Yoon; Chunduri, Prasad; Jo, Dong Gyu; Stranahan, Alexis Michelle; Mattson, Mark P.; Arumugam, Thiruma V.

In: Experimental Neurology, Vol. 257, 01.01.2014, p. 114-119.

Research output: Contribution to journalArticle

Fann, DYW, Santro, T, Manzanero, S, Widiapradja, A, Cheng, YL, Lee, SY, Chunduri, P, Jo, DG, Stranahan, AM, Mattson, MP & Arumugam, TV 2014, 'Intermittent fasting attenuates inflammasome activity in ischemic stroke', Experimental Neurology, vol. 257, pp. 114-119. https://doi.org/10.1016/j.expneurol.2014.04.017
Fann DYW, Santro T, Manzanero S, Widiapradja A, Cheng YL, Lee SY et al. Intermittent fasting attenuates inflammasome activity in ischemic stroke. Experimental Neurology. 2014 Jan 1;257:114-119. https://doi.org/10.1016/j.expneurol.2014.04.017
Fann, David Yang Wei ; Santro, Tomislav ; Manzanero, Silvia ; Widiapradja, Alexander ; Cheng, Yi Lin ; Lee, Seung Yoon ; Chunduri, Prasad ; Jo, Dong Gyu ; Stranahan, Alexis Michelle ; Mattson, Mark P. ; Arumugam, Thiruma V. / Intermittent fasting attenuates inflammasome activity in ischemic stroke. In: Experimental Neurology. 2014 ; Vol. 257. pp. 114-119.
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