Intestinal lipid absorption in the nephrotic rat

William D. Paulson, Carlos N. Torres-Rivera, Laura Gray, Patrick Tso

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

There is limited data on intestinal lipid absorption in the nephrotic syndrome. This study investigated whether the efficiency of intestinal lipid absorption is altered in nephrotic lymph-fistula rats. The nephrotic syndrome was induced in nine Sprague-Dawley rats by an iv injection of puromycin aminonucleoside in saline; seven control rats received saline only. At 10 to 14 days after injection, the main intestinal lymph duct was cannulated for collection of lymph. The duodenum was also cannulated and a fasting saline-glucose solution was infused overnight at 3 mL/h, The next day, the infusate was changed to a lipid emulsion that contained (14C)cholesterol and (3H)triglyceride (triolein) that was infused at 3 mL/h for 8 h. During the last hour of tasting and during the lipid infusion, lymph flow in the Nephrotic group averaged 0.6 mL/h higher than the Control group (P = 0.02). No significant differences were found between the two groups in recovery of infused radioactive cholesterol (P = 0.37) or triglyceride (P = 0.38) from the gastrointestinal lumen, small intestinal mucosa, or lymph. Lymphatic output of chemically measured cholesterol was also similar (P = 0.96). These results suggest that mucosal uptake and lymphatic output of cholesterol and triglyceride are not altered in the nephrotic syndrome.

Original languageEnglish (US)
Pages (from-to)431-436
Number of pages6
JournalJournal of the American Society of Nephrology
Volume7
Issue number3
StatePublished - Mar 1 1996

Keywords

  • Hypercholesterolemia
  • Hyperlipidemia
  • Hypertriglyceridemia
  • Puromycin aminonucleoside
  • Small intestine

ASJC Scopus subject areas

  • Nephrology

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