Intracavernosal basic fibroblast growth factor improves vasoreactivity in the hypercholesterolemic rabbit

Donghua Xie, Anne M. Pippen, Shelley I. Odronic, Brian H. Annex, Craig F. Donatucci

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Purpose. We determined the effects of intracavernosal injection (ICI) of recombinant basic fibroblast growth factor (rbFGF) on corporal tissue in hypercholesterolemic rabbits. Methods. Twenty New Zealand White rabbits were fed a 1% cholesterol diet for 6 weeks and were randomly divided into four groups. Group 1 (N = 5) received an ICI of phosphate buffered saline solution (PBS) once and again 3 weeks later. Group 2 (N = 4) received an ICI of 2.5 μg rbFGF once and PBS 3 weeks later. Group 3 (N = 6) received an ICI of 2.5 μg rbFGF once and again 3 weeks later. Group 4 (N = 5) received an ICI of 2.5 μg rbFGF once. All animals were maintained on the high cholesterol diet until sacrifice, 3 weeks after last injection. Strips of corporal tissue were submaximally contracted with norepinephrine, and dose-response curves were generated to evaluate endothelial-dependent (acetylcholine, ACH) and endothelial-independent (sodium nitroprusside, SNP) vasoreactivity. Protein levels of bFGF and vascular endothelial growth factor (VEGF) were assessed by enzyme-linked immunosorbent assay. Neuronal nitric oxide synthase (nNOS) protein and mRNA were detected by Western blot and semi-quantitative polymerase chain reaction, respectively. Results. Vasoreactivity was improved by bFGF treatment as shown by higher ED50[-log(M)] of ACH and SNP in Groups 2, 3, and 4. The expression of bFGF protein, VEGF protein, nNOS protein, and mRNA were all increased after bFGF treatment. Conclusion. ICI of bFGF improved vasoreactivity in hypercholesterolemic rabbit corporal tissue, offering a new direction to explore for the treatment of erectile dysfunction.

Original languageEnglish (US)
Pages (from-to)223-232
Number of pages10
JournalJournal of Sexual Medicine
Volume3
Issue number2
DOIs
StatePublished - Mar 2006
Externally publishedYes

Keywords

  • Angiogenic growth factors
  • Animal models
  • Endothelium
  • Fibrosis
  • Impotence
  • Penis
  • Pharmacologic studies in sexual function
  • Vascular physiologic studies of genital arousal

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Urology

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