Intracellular actions of 5‐hydroxytryptamine on the bivalve myocardium. II. Cyclic nucleotide‐dependent protein kinases and microsomal calcium uptake

William J. Higgins, Michael J. Greenberg

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effects of cyclic AMP, cyclic AMP‐induced phosphorylation, and 5‐hydroxytryptamine (5HT) on calcium uptake by bivalve heart microsomes were investigated. Cyclic AMP‐dependent and cyclic GMP‐dependent protein kinases were purified from Macrocallista nimbosa ventricles. The cyclic AMP‐dependent preparation readily phosphorylates myocardial cell proteins in both the soluble and microsomal fractions. The cyclic AMP‐mediated phosphorylation of microsomal protein augments microsomal calcium uptake; but 5HT and cyclic AMP also induce a similar increase directly. The protein kinase activity indigenous to the microsomal preparation was not increased by cyclic AMP. In many molluscan ventricles, 5HT increases intracellular cyclic AMP and simultaneously enhances muscle contractility by altering the amount of intracellular calcium available for contraction. Therefore, if cyclic AMP acts as a second messenger and mediates the effects of 5HT, the nucleotide should augment, directly or indirectly, the amount of membrane associated calcium. This study suggests that cyclic AMP can mediate 5HT action either by the activation of a cyclic AMP‐dependent protein kinase, or by a direct modification of the amount of membrane associated calcium.

Original languageEnglish (US)
Pages (from-to)305-315
Number of pages11
JournalJournal of Experimental Zoology
Volume190
Issue number3
DOIs
StatePublished - Dec 1974

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cyclic AMP
myocardium
protein kinases
Bivalvia
uptake mechanisms
calcium
phosphorylation
muscle strength
second messengers
microsomes
proteins
nucleotides
heart
cells

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Intracellular actions of 5‐hydroxytryptamine on the bivalve myocardium. II. Cyclic nucleotide‐dependent protein kinases and microsomal calcium uptake. / Higgins, William J.; Greenberg, Michael J.

In: Journal of Experimental Zoology, Vol. 190, No. 3, 12.1974, p. 305-315.

Research output: Contribution to journalArticle

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abstract = "The effects of cyclic AMP, cyclic AMP‐induced phosphorylation, and 5‐hydroxytryptamine (5HT) on calcium uptake by bivalve heart microsomes were investigated. Cyclic AMP‐dependent and cyclic GMP‐dependent protein kinases were purified from Macrocallista nimbosa ventricles. The cyclic AMP‐dependent preparation readily phosphorylates myocardial cell proteins in both the soluble and microsomal fractions. The cyclic AMP‐mediated phosphorylation of microsomal protein augments microsomal calcium uptake; but 5HT and cyclic AMP also induce a similar increase directly. The protein kinase activity indigenous to the microsomal preparation was not increased by cyclic AMP. In many molluscan ventricles, 5HT increases intracellular cyclic AMP and simultaneously enhances muscle contractility by altering the amount of intracellular calcium available for contraction. Therefore, if cyclic AMP acts as a second messenger and mediates the effects of 5HT, the nucleotide should augment, directly or indirectly, the amount of membrane associated calcium. This study suggests that cyclic AMP can mediate 5HT action either by the activation of a cyclic AMP‐dependent protein kinase, or by a direct modification of the amount of membrane associated calcium.",
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