Intracellular and extracellular calcium utilization during hypoxic vasoconstriction of cyclostome aortas

M. J. Russell, N. J. Pelaez, C. S. Packer, M. E. Forster, K. R. Olson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Hypoxic vasoconstriction (HV) is an intrinsic response of mammalian pulmonary and cyclostome aortic vascular smooth muscle. The present study examined the utilization of calcium during HV in dorsal aortas (DA) from sea lamprey and New Zealand hagfish. HV was temporally correlated with increased free cytosolic calcium (Ca2+) in lamprey DA. Extracellular calcium (Cao2+) did not contribute significantly to HV in lamprey DA, but it accounted for 38.1 ± 5.3% of HV in hagfish DA, Treatment of lamprey DA with ionomycin, ryanodine, or caffeine added to thapsigargin-reduced HV, whereas HV was augmented by BAY K 8644. Methoxyverapamil (D600) in zero Cao2+ did not affect HV in lamprey DA, nor did it prevent further constriction when Ca2+ was restored during hypoxia in hagfish DA. Removal of extracellular sodium (Nao+) caused a constriction in both species. Lamprey DA relaxed to prehypoxic tension following return to normoxia in zero Nao+, whereas relaxation was inhibited in hagfish DA. Relaxation following HV was inhibited in lamprey DA when Nao+ and Cao2+ were removed. These results show that HV is correlated with [Ca2+]c in lamprey DA and that Na+/Ca2+ exchange is used during HV in hagfish but not lamprey DA. Multiple receptor types appear to mediate stored intracellular calcium release in lamprey DA, and L-type calcium channels do not contribute significantly to constriction in either cyclostome.

Original languageEnglish (US)
Pages (from-to)R1506-R1513
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume281
Issue number5 50-5
StatePublished - Dec 17 2001

Keywords

  • Hagfish
  • Lamprey
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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