Intracellular kinases mediate increased translation and secretion of netrin-1 from renal tubular epithelial cells

Calpurnia Jayakumar, Riyaz Mohamed, Punithavathi Vilapakkam Ranganathan, Ganesan Ramesh

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Netrin-1 is a laminin-related secreted protein, is highly induced after tissue injury, and may serve as a marker of injury. However, the regulation of netrin-1 production is not unknown. Current study was carried out in mouse and mouse kidney cell line (TKPTS) to determine the signaling pathways that regulate netrin-1 production in response to injury. Methods and Principal Findings: Ischemia reperfusion injury of the kidney was induced in mice by clamping renal pedicle for 30 minutes. Cellular stress was induced in mouse proximal tubular epithelial cell line by treating with pervanadate, cisplatin, lipopolysaccharide, glucose or hypoxia followed by reoxygenation. Netrin-1 expression was quantified by real time RT-PCR and protein production was quantified using an ELISA kit. Cellular stress induced a large increase in netrin-1 production without increase in transcription of netrin-1 gene. Mitogen activated protein kinase, ERK mediates the drug induced netrin-1 mRNA translation increase without altering mRNA stability. Conclusion: Our results suggest that netrin-1 expression is suppressed at the translational level and MAPK activation leads to rapid translation of netrin-1 mRNA in the kidney tubular epithelial cells.

Original languageEnglish (US)
Article numbere26776
JournalPloS one
Volume6
Issue number10
DOIs
StatePublished - Nov 2 2011

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kidney cells
translation (genetics)
phosphotransferases (kinases)
epithelial cells
Phosphotransferases
Epithelial Cells
secretion
Kidney
mice
mitogen-activated protein kinase
cell lines
kidneys
laminin
cisplatin
ischemia
lipopolysaccharides
hypoxia
Messenger RNA
proteins
transcription (genetics)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Intracellular kinases mediate increased translation and secretion of netrin-1 from renal tubular epithelial cells. / Jayakumar, Calpurnia; Mohamed, Riyaz; Ranganathan, Punithavathi Vilapakkam; Ramesh, Ganesan.

In: PloS one, Vol. 6, No. 10, e26776, 02.11.2011.

Research output: Contribution to journalArticle

Jayakumar, Calpurnia ; Mohamed, Riyaz ; Ranganathan, Punithavathi Vilapakkam ; Ramesh, Ganesan. / Intracellular kinases mediate increased translation and secretion of netrin-1 from renal tubular epithelial cells. In: PloS one. 2011 ; Vol. 6, No. 10.
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