Intracellular trehalose improves the survival of cryopreserved mammalian cells

Ali Eroglu, Michael J. Russo, Robert Bieganski, Alex Fowler, Stephen Cheley, Hagan Bayley, Mehmet Toner

Research output: Contribution to journalArticle

348 Citations (Scopus)

Abstract

We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus α-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.

Original languageEnglish (US)
Pages (from-to)163-167
Number of pages5
JournalNature Biotechnology
Volume18
Issue number2
DOIs
StatePublished - Feb 1 2000

Fingerprint

Trehalose
Cells
Hemolysin Proteins
Cryopreservation
Fibroblasts
Keratinocytes
Freezing
Sugars
Staphylococcus aureus
Cell Survival
Membranes
Survival

Keywords

  • Cryopreservation
  • Fibroblasts
  • Keratinocytes
  • Trehalose
  • α-toxin

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this

Eroglu, A., Russo, M. J., Bieganski, R., Fowler, A., Cheley, S., Bayley, H., & Toner, M. (2000). Intracellular trehalose improves the survival of cryopreserved mammalian cells. Nature Biotechnology, 18(2), 163-167. https://doi.org/10.1038/72608

Intracellular trehalose improves the survival of cryopreserved mammalian cells. / Eroglu, Ali; Russo, Michael J.; Bieganski, Robert; Fowler, Alex; Cheley, Stephen; Bayley, Hagan; Toner, Mehmet.

In: Nature Biotechnology, Vol. 18, No. 2, 01.02.2000, p. 163-167.

Research output: Contribution to journalArticle

Eroglu, A, Russo, MJ, Bieganski, R, Fowler, A, Cheley, S, Bayley, H & Toner, M 2000, 'Intracellular trehalose improves the survival of cryopreserved mammalian cells', Nature Biotechnology, vol. 18, no. 2, pp. 163-167. https://doi.org/10.1038/72608
Eroglu A, Russo MJ, Bieganski R, Fowler A, Cheley S, Bayley H et al. Intracellular trehalose improves the survival of cryopreserved mammalian cells. Nature Biotechnology. 2000 Feb 1;18(2):163-167. https://doi.org/10.1038/72608
Eroglu, Ali ; Russo, Michael J. ; Bieganski, Robert ; Fowler, Alex ; Cheley, Stephen ; Bayley, Hagan ; Toner, Mehmet. / Intracellular trehalose improves the survival of cryopreserved mammalian cells. In: Nature Biotechnology. 2000 ; Vol. 18, No. 2. pp. 163-167.
@article{51a26ded85404af5b405069ef89766e0,
title = "Intracellular trehalose improves the survival of cryopreserved mammalian cells",
abstract = "We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus α-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80{\%} of 3T3 fibroblasts and 70{\%} of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.",
keywords = "Cryopreservation, Fibroblasts, Keratinocytes, Trehalose, α-toxin",
author = "Ali Eroglu and Russo, {Michael J.} and Robert Bieganski and Alex Fowler and Stephen Cheley and Hagan Bayley and Mehmet Toner",
year = "2000",
month = "2",
day = "1",
doi = "10.1038/72608",
language = "English (US)",
volume = "18",
pages = "163--167",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "Nature Publishing Group",
number = "2",

}

TY - JOUR

T1 - Intracellular trehalose improves the survival of cryopreserved mammalian cells

AU - Eroglu, Ali

AU - Russo, Michael J.

AU - Bieganski, Robert

AU - Fowler, Alex

AU - Cheley, Stephen

AU - Bayley, Hagan

AU - Toner, Mehmet

PY - 2000/2/1

Y1 - 2000/2/1

N2 - We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus α-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.

AB - We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus α-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.

KW - Cryopreservation

KW - Fibroblasts

KW - Keratinocytes

KW - Trehalose

KW - α-toxin

UR - http://www.scopus.com/inward/record.url?scp=0033951109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033951109&partnerID=8YFLogxK

U2 - 10.1038/72608

DO - 10.1038/72608

M3 - Article

C2 - 10657121

AN - SCOPUS:0033951109

VL - 18

SP - 163

EP - 167

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 2

ER -