Intracellular trehalose improves the survival of cryopreserved mammalian cells

Ali Eroglu; Michael J. Russo; Robert Bieganski; Alex Fowler; Stephen Cheley; Hagan Bayley; Mehmet Toner

doi:10.1038/72608

Intracellular trehalose improves the survival of cryopreserved mammalian cells

Ali Eroglu, Michael J. Russo, Robert Bieganski, Alex Fowler, Stephen Cheley, Hagan Bayley, Mehmet Toner

Research output: Contribution to journal › Article › peer-review

436 Scopus citations

Abstract

We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus α-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.

Original language	English (US)
Pages (from-to)	163-167
Number of pages	5
Journal	Nature Biotechnology
Volume	18
Issue number	2
DOIs	https://doi.org/10.1038/72608
State	Published - Feb 2000
Externally published	Yes

Keywords

Cryopreservation
Fibroblasts
Keratinocytes
Trehalose
α-toxin

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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10.1038/72608

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title = "Intracellular trehalose improves the survival of cryopreserved mammalian cells",

abstract = "We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus α-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.",

keywords = "Cryopreservation, Fibroblasts, Keratinocytes, Trehalose, α-toxin",

author = "Ali Eroglu and Russo, {Michael J.} and Robert Bieganski and Alex Fowler and Stephen Cheley and Hagan Bayley and Mehmet Toner",

note = "Funding Information: This work was partially supported by the National Institutes of Health grants DK46270 and NS26760, Shriners Hospitals for Children, ONR and DOE. The authors thank Xiaofeng Lu for technical assistance. The authors also acknowledge Dr. Jeffrey Morgan{\textquoteright}s assistance in keratinocyte culture and evaluation. Michael J. Russo was a Howard Hughes Medical Institute PreDoctoral Fellow.",

year = "2000",

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doi = "10.1038/72608",

language = "English (US)",

volume = "18",

pages = "163--167",

journal = "Nature Biotechnology",

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T1 - Intracellular trehalose improves the survival of cryopreserved mammalian cells

AU - Eroglu, Ali

AU - Russo, Michael J.

AU - Bieganski, Robert

AU - Fowler, Alex

AU - Cheley, Stephen

AU - Bayley, Hagan

AU - Toner, Mehmet

N1 - Funding Information: This work was partially supported by the National Institutes of Health grants DK46270 and NS26760, Shriners Hospitals for Children, ONR and DOE. The authors thank Xiaofeng Lu for technical assistance. The authors also acknowledge Dr. Jeffrey Morgan’s assistance in keratinocyte culture and evaluation. Michael J. Russo was a Howard Hughes Medical Institute PreDoctoral Fellow.

PY - 2000/2

Y1 - 2000/2

N2 - We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus α-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.

AB - We report that the introduction of low concentrations of intracellular trehalose can greatly improve the survival of mammalian cells during cryopreservation. Using a genetically engineered mutant of Staphylococcus aureus α-hemolysin to create pores in the cellular membrane, we were able to load trehalose into cells. Low concentrations (0.2 M) of trehalose permitted long-term post-thaw survival of more than 80% of 3T3 fibroblasts and 70% of human keratinocytes. These results indicate that simplified and widely applicable freezing protocols may be possible using sugars as intracellular cryoprotective additives.

KW - Cryopreservation

KW - Fibroblasts

KW - Keratinocytes

KW - Trehalose

KW - α-toxin

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JF - Nature Biotechnology

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ER -

Intracellular trehalose improves the survival of cryopreserved mammalian cells

Abstract

Keywords

ASJC Scopus subject areas

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