Intracerebral xenografts of mouse bone marrow cells in adult rats facilitate restoration of cerebral blood flow and blood-brain barrier

Cesario V. Borlongan, Jeffrey G. Lind, Ora Dillon-Carter, Guolong Yu, Martin Hadman, Charles Cheng, James Edwin Carroll, David C Hess

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

We examined in the present study alterations in cerebral blood flow (CBF) and blood-brain barrier (BBB) permeability following intrastriatal transplantation of mouse bone marrow stromal cells (BMSCs) or saline infusion in adult Sprague-Dawley rats. Laser Doppler revealed that transplanted animals exhibited near normal cerebral blood flow (CBF, 150 perfusion units) at a much earlier period post-transplantation (day 4) compared to animals that received saline infusion (day 12) (p's<0.05). Similarly, Evans Blue assay demonstrated that transplanted animals exhibited near complete BBB reconstitution at day 5 post-transplantation, whereas animals that received saline infusion continued to display a compromised BBB up to 11 days post-transplantation. Transplanted animals displayed a cell dose-dependent CBF and BBB restoration. Enzyme-linked immunosorbent assay (ELISA) of transplanted BMSCs revealed elevated levels of transforming growth factor-beta superfamily of neurotrophic factors. Moreover, despite the absence of immunosuppression in this cross-species transplantation, at least in the acute phase (12 days post-transplantation), surviving xenografts were detected during periods of restored CBF and BBB permeability. These observations suggest that restoration of CBF and BBB permeability accompanies the reported functional outcomes associated with intracerebral transplantation of BMSCs.

Original languageEnglish (US)
Pages (from-to)26-33
Number of pages8
JournalBrain Research
Volume1009
Issue number1-2
DOIs
StatePublished - May 29 2004

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Cerebrovascular Circulation
Blood-Brain Barrier
Heterografts
Bone Marrow Cells
Transplantation
Mesenchymal Stromal Cells
Permeability
Evans Blue
Heterologous Transplantation
Nerve Growth Factors
Transforming Growth Factor beta
Immunosuppression
Sprague Dawley Rats
Lasers
Perfusion
Enzyme-Linked Immunosorbent Assay

Keywords

  • Cross-species
  • Development and regeneration
  • Immunosuppression
  • Neural transplantation
  • Neuroprotection
  • Stem cell
  • Striatum
  • Transplantation

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Intracerebral xenografts of mouse bone marrow cells in adult rats facilitate restoration of cerebral blood flow and blood-brain barrier. / Borlongan, Cesario V.; Lind, Jeffrey G.; Dillon-Carter, Ora; Yu, Guolong; Hadman, Martin; Cheng, Charles; Carroll, James Edwin; Hess, David C.

In: Brain Research, Vol. 1009, No. 1-2, 29.05.2004, p. 26-33.

Research output: Contribution to journalArticle

Borlongan, Cesario V. ; Lind, Jeffrey G. ; Dillon-Carter, Ora ; Yu, Guolong ; Hadman, Martin ; Cheng, Charles ; Carroll, James Edwin ; Hess, David C. / Intracerebral xenografts of mouse bone marrow cells in adult rats facilitate restoration of cerebral blood flow and blood-brain barrier. In: Brain Research. 2004 ; Vol. 1009, No. 1-2. pp. 26-33.
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