Intrafibrillar silicification of collagen scaffolds for sustained release of stem cell homing chemokine in hard tissue regeneration

Li Na Niu, Kai Jiao, Yi Pin Qi, Sergey Nikonov, Cynthia K.Y. Yiu, Dwayne D. Arola, Shi Qiang Gong, Ahmed El-Marakby, Marcela R.O. Carrilho, Mark W. Hamrick, Kenneth M. Hargreaves, Anibal Diogenes, Ji Hua Chen, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Traditional bone regeneration strategies relied on supplementation of biomaterials constructs with stem or progenitor cells or growth factors. By contrast, cell homing strategies employ chemokines to mobilize stem or progenitor cells from host bone marrow and tissue niches to injured sites. Although silica-based biomaterials exhibit osteogenic and angiogenic potentials, they lack cell homing capability. Stromal cell-derived factor-1 (SDF-1) plays a pivotal role in mobilization and homing of stem cells to injured tissues. In this work, we demonstrated that 3-dimensional collagen scaffolds infiltrated with intrafibrillar silica are biodegradable and highly biocompatible. They exhibit improved compressive stress-strain responses and toughness over nonsilicified collagen scaffolds. They are osteoconductive and up-regulate expressions of osteogenesis- and angiogenesis-related genes more significantly than nonsilicified collagen scaffolds. In addition, these scaffolds reversibly bind SDF-1α for sustained release of this chemokine, which exhibits in vitro cell homing characteristics. When implanted subcutaneously in an in vivo mouse model, SDF-1α-loaded silicified collagen scaffolds stimulate the formation of ectopic bone and blood capillaries within the scaffold and abrogate the need for cell seeding or supplementation of osteogenic and angiogenic growth factors. Intrafibrillar-silicified collagen scaffolds with sustained SDF-1α release represent a less costly and complex alternative to contemporary cell seeding approaches and provide new therapeutic options for in situ hard tissue regeneration.

Original languageEnglish (US)
Pages (from-to)4517-4529
Number of pages13
JournalFASEB Journal
Volume26
Issue number11
DOIs
StatePublished - Nov 1 2012

Fingerprint

Tissue regeneration
Stem cells
Chemokines
Scaffolds
Regeneration
Collagen
Stem Cells
Chemokine CXCL12
Biocompatible Materials
Osteogenesis
Silicon Dioxide
Bone
Intercellular Signaling Peptides and Proteins
Hematopoietic Stem Cell Mobilization
Bone Regeneration
Angiogenesis Inducing Agents
Tissue
Up-Regulation
Bone Marrow
Compressive stress

Keywords

  • Angiogenesis
  • Bone regeneration
  • Chemotaxis
  • Osteogenesis

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Intrafibrillar silicification of collagen scaffolds for sustained release of stem cell homing chemokine in hard tissue regeneration. / Niu, Li Na; Jiao, Kai; Qi, Yi Pin; Nikonov, Sergey; Yiu, Cynthia K.Y.; Arola, Dwayne D.; Gong, Shi Qiang; El-Marakby, Ahmed; Carrilho, Marcela R.O.; Hamrick, Mark W.; Hargreaves, Kenneth M.; Diogenes, Anibal; Chen, Ji Hua; Pashley, David H.; Tay, Franklin R.

In: FASEB Journal, Vol. 26, No. 11, 01.11.2012, p. 4517-4529.

Research output: Contribution to journalArticle

Niu, LN, Jiao, K, Qi, YP, Nikonov, S, Yiu, CKY, Arola, DD, Gong, SQ, El-Marakby, A, Carrilho, MRO, Hamrick, MW, Hargreaves, KM, Diogenes, A, Chen, JH, Pashley, DH & Tay, FR 2012, 'Intrafibrillar silicification of collagen scaffolds for sustained release of stem cell homing chemokine in hard tissue regeneration', FASEB Journal, vol. 26, no. 11, pp. 4517-4529. https://doi.org/10.1096/fj.12-210211
Niu, Li Na ; Jiao, Kai ; Qi, Yi Pin ; Nikonov, Sergey ; Yiu, Cynthia K.Y. ; Arola, Dwayne D. ; Gong, Shi Qiang ; El-Marakby, Ahmed ; Carrilho, Marcela R.O. ; Hamrick, Mark W. ; Hargreaves, Kenneth M. ; Diogenes, Anibal ; Chen, Ji Hua ; Pashley, David H. ; Tay, Franklin R. / Intrafibrillar silicification of collagen scaffolds for sustained release of stem cell homing chemokine in hard tissue regeneration. In: FASEB Journal. 2012 ; Vol. 26, No. 11. pp. 4517-4529.
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AU - Yiu, Cynthia K.Y.

AU - Arola, Dwayne D.

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AU - El-Marakby, Ahmed

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AU - Hargreaves, Kenneth M.

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