Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration

Jin long Sun, Kai Jiao, Li na Niu, Yang Jiao, Qun Song, Li juan Shen, Franklin Chi Meng Tay, Ji hua Chen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The immunomodulatory functions of monocytes are increasingly being recognized. Silicified collagen scaffolds (SCSs), produced by infiltrating collagen matrices with intrafibrillar amorphous silica, exhibit osteogenic and angiogenic potential and are promising candidates in tissue engineering. Here, we demonstrate that SCS promotes in situ bone regeneration and angiogenesis via monocyte immunomodulation. Increased numbers of TRAP-positive monocytes, nestin-positive bone marrow stromal cells (BMSCs) and CD31-positive and endomucin-positive new vessels can be identified from new bone formation regions in a murine calvarial defect model. In addition, sustained release of silicic acid by SCS stimulates differentiation of blood-derived monocytes into TRAP-positive cells, with increased expressions of SDF-1α, TGF-β1, VEGFa and PDGF-BB. These cytokines further promote homing of BMSCs and endothelial progenitor cells as well as neovascularization. Taken together, these novel findings indicate that SCSs possess the ability to enhance recruitment of progenitor cells and promote osteogenesis and angiogenesis by immunomodulation of monocytes.

Original languageEnglish (US)
Pages (from-to)203-216
Number of pages14
JournalBiomaterials
Volume113
DOIs
StatePublished - Jan 1 2017

Fingerprint

Bone Regeneration
Scaffolds (biology)
Collagen
Scaffolds
Monocytes
Bone
Immunomodulation
Mesenchymal Stromal Cells
Osteogenesis
Sialomucins
Silicic Acid
Nestin
Bioelectric potentials
Endothelial cells
Tissue Engineering
Tissue engineering
Silicon Dioxide
Blood
Stem Cells
Silica

Keywords

  • Angiogenesis
  • Bone regeneration
  • Cell homing
  • Immunomodulation
  • Intrafibrillar silicification
  • Monocytes

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration. / Sun, Jin long; Jiao, Kai; Niu, Li na; Jiao, Yang; Song, Qun; Shen, Li juan; Tay, Franklin Chi Meng; Chen, Ji hua.

In: Biomaterials, Vol. 113, 01.01.2017, p. 203-216.

Research output: Contribution to journalArticle

Sun, Jin long ; Jiao, Kai ; Niu, Li na ; Jiao, Yang ; Song, Qun ; Shen, Li juan ; Tay, Franklin Chi Meng ; Chen, Ji hua. / Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration. In: Biomaterials. 2017 ; Vol. 113. pp. 203-216.
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