Abstract
The immunomodulatory functions of monocytes are increasingly being recognized. Silicified collagen scaffolds (SCSs), produced by infiltrating collagen matrices with intrafibrillar amorphous silica, exhibit osteogenic and angiogenic potential and are promising candidates in tissue engineering. Here, we demonstrate that SCS promotes in situ bone regeneration and angiogenesis via monocyte immunomodulation. Increased numbers of TRAP-positive monocytes, nestin-positive bone marrow stromal cells (BMSCs) and CD31-positive and endomucin-positive new vessels can be identified from new bone formation regions in a murine calvarial defect model. In addition, sustained release of silicic acid by SCS stimulates differentiation of blood-derived monocytes into TRAP-positive cells, with increased expressions of SDF-1α, TGF-β1, VEGFa and PDGF-BB. These cytokines further promote homing of BMSCs and endothelial progenitor cells as well as neovascularization. Taken together, these novel findings indicate that SCSs possess the ability to enhance recruitment of progenitor cells and promote osteogenesis and angiogenesis by immunomodulation of monocytes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 203-216 |
| Number of pages | 14 |
| Journal | Biomaterials |
| Volume | 113 |
| DOIs | |
| State | Published - Jan 1 2017 |
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Keywords
- Angiogenesis
- Bone regeneration
- Cell homing
- Immunomodulation
- Intrafibrillar silicification
- Monocytes
ASJC Scopus subject areas
- Bioengineering
- Ceramics and Composites
- Biophysics
- Biomaterials
- Mechanics of Materials
Cite this
Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration. / Sun, Jin long; Jiao, Kai; Niu, Li na; Jiao, Yang; Song, Qun; Shen, Li juan; Tay, Franklin R.; Chen, Ji hua.
In: Biomaterials, Vol. 113, 01.01.2017, p. 203-216.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration
AU - Sun, Jin long
AU - Jiao, Kai
AU - Niu, Li na
AU - Jiao, Yang
AU - Song, Qun
AU - Shen, Li juan
AU - Tay, Franklin R.
AU - Chen, Ji hua
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The immunomodulatory functions of monocytes are increasingly being recognized. Silicified collagen scaffolds (SCSs), produced by infiltrating collagen matrices with intrafibrillar amorphous silica, exhibit osteogenic and angiogenic potential and are promising candidates in tissue engineering. Here, we demonstrate that SCS promotes in situ bone regeneration and angiogenesis via monocyte immunomodulation. Increased numbers of TRAP-positive monocytes, nestin-positive bone marrow stromal cells (BMSCs) and CD31-positive and endomucin-positive new vessels can be identified from new bone formation regions in a murine calvarial defect model. In addition, sustained release of silicic acid by SCS stimulates differentiation of blood-derived monocytes into TRAP-positive cells, with increased expressions of SDF-1α, TGF-β1, VEGFa and PDGF-BB. These cytokines further promote homing of BMSCs and endothelial progenitor cells as well as neovascularization. Taken together, these novel findings indicate that SCSs possess the ability to enhance recruitment of progenitor cells and promote osteogenesis and angiogenesis by immunomodulation of monocytes.
AB - The immunomodulatory functions of monocytes are increasingly being recognized. Silicified collagen scaffolds (SCSs), produced by infiltrating collagen matrices with intrafibrillar amorphous silica, exhibit osteogenic and angiogenic potential and are promising candidates in tissue engineering. Here, we demonstrate that SCS promotes in situ bone regeneration and angiogenesis via monocyte immunomodulation. Increased numbers of TRAP-positive monocytes, nestin-positive bone marrow stromal cells (BMSCs) and CD31-positive and endomucin-positive new vessels can be identified from new bone formation regions in a murine calvarial defect model. In addition, sustained release of silicic acid by SCS stimulates differentiation of blood-derived monocytes into TRAP-positive cells, with increased expressions of SDF-1α, TGF-β1, VEGFa and PDGF-BB. These cytokines further promote homing of BMSCs and endothelial progenitor cells as well as neovascularization. Taken together, these novel findings indicate that SCSs possess the ability to enhance recruitment of progenitor cells and promote osteogenesis and angiogenesis by immunomodulation of monocytes.
KW - Angiogenesis
KW - Bone regeneration
KW - Cell homing
KW - Immunomodulation
KW - Intrafibrillar silicification
KW - Monocytes
UR - http://www.scopus.com/inward/record.url?scp=84995644736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84995644736&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2016.10.050
DO - 10.1016/j.biomaterials.2016.10.050
M3 - Article
C2 - 27821306
AN - SCOPUS:84995644736
VL - 113
SP - 203
EP - 216
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
ER -